Evidence map›Paper›PMID 29157304›Full record

ArticleActa neuropathologica communications2017

A novel vector for transgenesis in the rat CNS.

T Peter Lopez, Kurt Giles, Brittany N Dugger, Abby Oehler, Carlo Condello, Zuzana Krejciova, Julian A Castaneda, George A Carlson, Stanley B Prusiner

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta neuropathologica communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

T Peter LopezInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Kurt GilesInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Brittany N DuggerInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Abby OehlerInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Carlo CondelloInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Zuzana KrejciovaInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Julian A CastanedaInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
George A CarlsonInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
Stanley B PrusinerInstitute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, Sandler Neurosciences Center, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA. stanley.prusiner@ucsf.edu.
University of California, San Francisco · US

Funding

VIRAL DEGENERATIVE DEMENTING DISEASE IN AGING (SUPP)P01AG002132 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SCHNIER, PAUL · 1985 to 2024
$51.7M
Towards Therapeutics for Neurodegenerative DiseasesR37AG031220 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PRUSINER, STANLEY B · 2008 to 2017
$6.2M
ANALYSES AND APPLICATIONS OF GLIAL FIBRILLARY ACIDIC PROR01NS039055 · NINDS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI BRENNER, MICHAEL · 1999 to 2010
$2.6M
Characterizing the Cellular Biology of Prion Strains and the Factors that Drive Prion PathogenesisF32NS095587 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOPEZ, TIMOTHY PETER · 2015 to 2018
$159k
NIA NIH HHS AG002132NIA NIH HHS AG031220NIA NIH HHS P01 AG002132NIA NIH HHS R37 AG031220NINDS NIH HHS F32 NS095587NINDS NIH HHS NS095587NINDS NIH HHS R01 NS039055
6 · The paper itself

Abstract

The larger brain of the rat enables a much greater repertoire of complex behaviors than mice, likely making rats preferential for investigating neurodegeneration. Because molecular tools for specific expression of transgenes in the rat brain are sparse, we chose Prnp encoding the prion protein (PrP) to develop a novel vector to drive transgene expression in the rat brain. We compared the rat Prnp sequence with mouse and Syrian hamster Prnp sequences, identifying conserved genetic elements and hypothesizing that these elements would be able to drive neuronal transgene expression. We investigated this by generating a vector termed RaPrnp that encompasses portions of the rat Prnp gene. Importantly, we replaced the rat Prnp open reading frame (ORF) with a cloning site for rapid and seamless In-Fusion cloning. To validate the in vivo neuronal specificity of the RaPrnp vector in rats, we generated stable RaPrnp-LacZ/enhanced green fluorescent protein (EGFP) transgenic (Tg) rat lines, which led to robust LacZ activity and high EGFP fluorescence in the central nervous system of embryos and adult animals. Next, we restored the rat Prnp ORF and generated multiple Tg(RaPrnp-PrP) lines, demonstrating that overexpression of Prnp accelerates the onset of scrapie. While the incubation time in wild-type (WT) rats was 175 ± 3 days post inoculation (dpi), one line, Tg2919, overexpressed RaPrP

Indexed as

Genetic VectorsGene Transfer TechniquesAnimalsAnimals, Genetically ModifiedAnimals, NewbornCalcium-Binding ProteinsCentral Nervous SystemCricetinaeDisease Models, AnimalEmbryo, MammalianGene Expression Regulation, DevelopmentalGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsMesocricetusMiceMicrofilament ProteinsAif1 protein, ratCalcium-Binding ProteinsGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsMicrofilament ProteinsPhosphopyruvate HydratasePrion ProteinsPRNP protein, humanNeurodegenerative diseasePrnpScrapieVector

Identifiers

PMID29157304
PMCPMC5697436
OpenAlexW2768797601

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.