Evidence map›Paper›PMID 29157299›Full record

ArticleStem cell research & therapy2017

The similarities between smDCs and regDCs in alleviating the immune injury caused by transplantation of hepatocytes differentiated from ESCs.

Cheng Zhang, Wenwei Liao, Bing Cai, Furong Liu, Qiong Ke, Xiaofeng Zhu, Xiaoshun He, Anbin Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 75% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Cheng ZhangOrgan Transplant Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology,Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, China.
Wenwei LiaoOrgan Transplant Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology,Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, China.
Bing CaiReproductive Medicine Center of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Furong LiuCardiac Surgery Itensive Care Unit of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Qiong KeZhongshan Medical School of Sun Yat-sen University, Guangzhou, China.
Xiaofeng ZhuOrgan Transplant Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology,Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, China.
Xiaoshun HeOrgan Transplant Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology,Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, China.
Anbin HuOrgan Transplant Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology,Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, China. anbinhu@foxmail.com.
Sun Yat-sen University · CN

Funding

National Natural Science Foundation of China 81672998, 30700398, 81172831 and 81371693Natural Science Foundation of Guangdong Province S2013010015935, 10151008901000200people's welfare scientific and technological projects of Guangzhou city 2060404, 2014Y2-00114scientific and technological projects of Guangdong 2017A010105016, 2013B010404016, 2012B031800257, 2013B051000020
6 · The paper itself

Abstract

backgroundThis study aimed to investigate the tolerogenic mechanisms induced by semimature dendritic cells (smDCs) and regulatory dendritic cells (regDCs) after transplantation of hepatocytes differentiated from mouse embryonic stem cells (ESCs) and to confirm the low immunogenicity of hepatocytes differentiated from ESCs.

methodsGreen fluorescent protein-labeled ESCs collected from 129 mice were cultured to differentiate into hepatocytes. smDCs and regDCs were cultured in vitro. The hepatocytes were cultured after being extracted from the livers of 129 mice. After injecting smDCs or regDCs 3 days in advance, these differentiated hepatocytes and normal hepatocytes were transplanted into the livers of BALB/c mice separately. Subsequently, the histopathological features and cytokines in transplant tissues as well as the Foxp3 expression in peripheral blood CD4

resultsThe morphological phenotypes of smDCs and regDCs were similar. They both expressed medium levels of MHC-II, CD40, CD80, and CD86, high levels of TGF-β and IL-10, and low levels of IL-2. The survival of differentiated hepatocytes was prolonged and inflammatory infiltration in transplant tissues was reduced in both the smDC and regDC groups. Foxp3 expression in peripheral blood CD4

conclusionssmDCs and regDCs were similar tolerogenic dendritic cells. They both could alleviate the immune injury by inducing CD4

Indexed as

Immune ToleranceAnimalsAntigens, CDBiomarkersCell DifferentiationDendritic CellsEmbryonic Stem CellsForkhead Transcription FactorsGene ExpressionGenes, ReporterGreen Fluorescent ProteinsHepatocytesInterleukin-10Interleukin-2MiceMice, Inbred BALB CAntigens, CDBiomarkersForkhead Transcription FactorsFoxp3 protein, mouseGreen Fluorescent ProteinsIL10 protein, mouseInterleukin-10Interleukin-2Transforming Growth Factor betaEmbryonic stem cellsHepatocyte transplantationLow immunogenicityRegulatory dendritic cellsRegulatory T cellsSemimature dendritic cells

Identifiers

PMID29157299
PMCPMC5696883
OpenAlexW2768136196

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.