ArticleBMC musculoskeletal disorders2017
A new multiple trauma model of the mouse.
Article in BMC musculoskeletal disorders, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 17 citations in OpenAlex.
- Fraxinellone mitigates acute lung injury by targeting HIF-1α to suppress pyroptosis and inflammation.Frontiers in immunology · 2026Article
- Systemic versus local delivery of mesenchymal stem cells to improve the early stages of fracture healing in a polytrauma model.Journal of biological engineering · 2025Article
- Experimental study of a novel mouse model of tibial shaft fracture combined with blunt chest trauma.Animal models and experimental medicine · 2025Article
- Polytrauma impairs fracture healing accompanied by increased persistence of innate inflammatory stimuli and reduced adaptive response.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Mast Cells Drive Systemic Inflammation and Compromised Bone Repair After Trauma.Frontiers in immunology · 2022Article
- The future of basic science in orthopaedics and traumatology: Cassandra or Prometheus?European journal of medical research · 2021Review
- Different experimental multiple trauma models induce comparable inflammation and organ injury.Scientific reports · 2020Article
- Experimental induction of blunt chest trauma in mice: A modified approach with evaluation in dummies and cadavers.Experimental and therapeutic medicine · 2020Article
- Review
- Intact Glucocorticoid Receptor Dimerization Is Deleterious in Trauma-Induced Impaired Fracture Healing.Frontiers in immunology · 2020Article
- Traumatic injury pattern is of equal relevance as injury severity for experimental (poly)trauma modeling.Scientific reports · 2019Article
- Correction to: A new multiple trauma model of the mouse.BMC musculoskeletal disorders · 2019Article
- Development, Validation and Characterization of a Novel Portable Closed Fracture Device.In vivo (Athens, Greece)Article
- Mesenchymal stem cells delivered via a bioactive disordered peptide-hydrogel platform modulate early inflammation and enhance skeletal repair in a polytrauma model.Journal of tissue engineeringArticle
- The Role of Macrophages During Mammalian Tissue Remodeling and Regeneration Under Infectious and Non-Infectious Conditions.Frontiers in immunologyReview
Corrections and comments
- Erratum issued
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBlunt trauma is the most frequent mechanism of injury in multiple trauma, commonly resulting from road traffic collisions or falls. Two of the most frequent injuries in patients with multiple trauma are chest trauma and extremity fracture. Several trauma mouse models combine chest trauma and head injury, but no trauma mouse model to date includes the combination of long bone fractures and chest trauma. Outcome is essentially determined by the combination of these injuries. In this study, we attempted to establish a reproducible novel multiple trauma model in mice that combines blunt trauma, major injuries and simple practicability.
methodsNinety-six male C57BL/6 N mice (n = 8/group) were subjected to trauma for isolated femur fracture and a combination of femur fracture and chest injury. Serum samples of mice were obtained by heart puncture at defined time points of 0 h (hour), 6 h, 12 h, 24 h, 3 d (days), and 7 d.
resultsA tendency toward reduced weight and temperature was observed at 24 h after chest trauma and femur fracture. Blood analyses revealed a decrease in hemoglobin during the first 24 h after trauma. Some animals were killed by heart puncture immediately after chest contusion; these animals showed the most severe lung contusion and hemorrhage. The extent of structural lung injury varied in different mice but was evident in all animals. Representative H&E-stained (Haematoxylin and Eosin-stained) paraffin lung sections of mice with multiple trauma revealed hemorrhage and an inflammatory immune response. Plasma samples of mice with chest trauma and femur fracture showed an up-regulation of IL-1β (Interleukin-1β), IL-6, IL-10, IL-12p70 and TNF-α (Tumor necrosis factor- α) compared with the control group. Mice with femur fracture and chest trauma showed a significant up-regulation of IL-6 compared to group with isolated femur fracture.
conclusionsThe multiple trauma mouse model comprising chest trauma and femur fracture enables many analogies to clinical cases of multiple trauma in humans and demonstrates associated characteristic clinical and pathophysiological changes. This model is easy to perform, is economical and can be used for further research examining specific immunological questions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.