ArticleBMC plant biology2017
Genome-wide identification of conserved and novel microRNAs in one bud and two tender leaves of tea plant (Camellia sinensis) by small RNA sequencing, microarray-based hybridization and genome survey scaffold sequences.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 94 citations in OpenAlex.
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- Revealing the mechanisms of the bioactive ingredients accumulation inFrontiers in plant science · 2022Article
- Sequence, Secondary Structure, and Phylogenetic Conservation of MicroRNAs inBioinformatics and biology insights · 2022Article
- Comparative transcriptome analysis reveals key pathways and genes involved in trichome development in tea plant (Frontiers in plant science · 2022Article
- Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid inInternational journal of molecular sciences · 2021Article
- Analysis of miRNA-mediated regulation of flowering induction in Lilium × formolongi.BMC plant biology · 2021Article
- Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [RNA biology · 2020Article
- Systematic Identification and Analysis of Light-Responsive Circular RNA and Co-expression Networks in Lettuce (G3 (Bethesda, Md.) · 2020Article
- Transgenerational Effects of Water-Deficit and Heat Stress on Germination and Seedling Vigour-New Insights from Durum Wheat microRNAs.Plants (Basel, Switzerland) · 2020Article
- Mining of miRNAs from EST data in Dendrobium nobile.Bioinformation · 2020Article
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7 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundMicroRNAs (miRNAs) are important for plant growth and responses to environmental stresses via post-transcriptional regulation of gene expression. Tea, which is primarily produced from one bud and two tender leaves of the tea plant (Camellia sinensis), is one of the most popular non-alcoholic beverages worldwide owing to its abundance of secondary metabolites. A large number of miRNAs have been identified in various plants, including non-model species. However, due to the lack of reference genome sequences and/or information of tea plant genome survey scaffold sequences, discovery of miRNAs has been limited in C. sinensis.
resultsUsing small RNA sequencing, combined with our recently obtained genome survey data, we have identified and analyzed 175 conserved and 83 novel miRNAs mainly in one bud and two tender leaves of the tea plant. Among these, 93 conserved and 18 novel miRNAs were validated using miRNA microarray hybridization. In addition, the expression pattern of 11 conserved and 8 novel miRNAs were validated by stem-loop-qRT-PCR. A total of 716 potential target genes of identified miRNAs were predicted. Further, Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that most of the target genes were primarily involved in stress response and enzymes related to phenylpropanoid biosynthesis. The predicted targets of 4 conserved miRNAs were further validated by 5'RLM-RACE. A negative correlation between expression profiles of 3 out of 4 conserved miRNAs (csn-miR160a-5p, csn-miR164a, csn-miR828 and csn-miR858a) and their targets (ARF17, NAC100, WER and MYB12 transcription factor) were observed.
conclusionIn summary, the present study is one of few such studies on miRNA detection and identification in the tea plant. The predicted target genes of majority of miRNAs encoded enzymes, transcription factors, and functional proteins. The miRNA-target transcription factor gene interactions may provide important clues about the regulatory mechanism of these miRNAs in the tea plant. The data reported in this study will make a huge contribution to knowledge on the potential miRNA regulators of the secondary metabolism pathway and other important biological processes in C. sinensis.
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