ArticleJournal of experimental botany2017
Transcript profiling of a bitter variety of narrow-leafed lupin to discover alkaloid biosynthetic genes.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Proteomics and Alkaloid Signature of Narrow Leaf Lupin Leaves Reveal Key Enzymes in Quinolizidine Alkaloid Biosynthesis and Transportation.Journal of agricultural and food chemistry · 2026Article
- Genomic structure of yellow lupin (Lupinus luteus): genome organization, evolution, gene family expansion, metabolites and protein synthesis.BMC genomics · 2025Article
- The aerial epidermis is a major site of quinolizidine alkaloid biosynthesis in narrow-leafed lupin.The New phytologist · 2025Article
- Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (-)-sparteine.Plant biotechnology journal · 2025Article
- Emerging functions within the enzyme families of plant alkaloid biosynthesis.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2023Article
- Review
- The causal mutation leading to sweetness in modern white lupin cultivars.Science advances · 2023Article
- A Pyridine Dearomatization Approach to the Matrine-Type Lupin Alkaloids.Journal of the American Chemical Society · 2022Article
- Insights into polyamine metabolism: homospermidine is double-oxidized in two discrete steps by a single copper-containing amine oxidase in pyrrolizidine alkaloid biosynthesis.The Plant cell · 2022Article
- Progress of Genomics-Driven Approaches for Sustaining Underutilized Legume Crops in the Post-Genomic Era.Frontiers in genetics · 2022Review
- Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin.Plant methods · 2021Article
- A metabolic regulon reveals early and late acting enzymes in neuroactive Lycopodium alkaloid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Expression Profiles of Alkaloid-Related Genes across the Organs of Narrow-Leafed Lupin (International journal of molecular sciences · 2021Article
- Plant Copper Metalloenzymes As Prospects for New Metabolism Involving Aromatic Compounds.Frontiers in plant science · 2021Review
- Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant 'Kushen' (Frontiers in plant science · 2021Article
- A Bitter-Sweet Story: Unraveling the Genes Involved in Quinolizidine Alkaloid Synthesis inFrontiers in plant science · 2021Review
- Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination.Plants (Basel, Switzerland) · 2019Article
- Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (International journal of molecular sciences · 2019Article
- Development of gene-based molecular markers tagging low alkaloid pauper locus in white lupin (Lupinus albus L.).Journal of applied genetics · 2019Article
- Metabolic diversification of nitrogen-containing metabolites by the expression of a heterologous lysine decarboxylase gene in Arabidopsis.The Plant journal : for cell and molecular biology · 2019Article
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8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Lupins (Lupinus spp.) are nitrogen-fixing legumes that accumulate toxic alkaloids in their protein-rich beans. These anti-nutritional compounds belong to the family of quinolizidine alkaloids (QAs), which are of interest to the pharmaceutical and chemical industries. To unleash the potential of lupins as protein crops and as sources of QAs, a thorough understanding of the QA pathway is needed. However, only the first enzyme in the pathway, lysine decarboxylase (LDC), is known. Here, we report the transcriptome of a high-QA variety of narrow-leafed lupin (L. angustifolius), obtained using eight different tissues and two different sequencing technologies. In addition, we present a list of 33 genes that are closely co-expressed with LDC and that represent strong candidates for involvement in lupin alkaloid biosynthesis. One of these genes encodes a copper amine oxidase able to convert the product of LDC, cadaverine, into 1-piperideine, as shown by heterologous expression and enzyme assays. Kinetic analysis revealed a low KM value for cadaverine, supporting a role as the second enzyme in the QA pathway. Our transcriptomic data set represents a crucial step towards the discovery of enzymes, transporters, and regulators involved in lupin alkaloid biosynthesis.
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