ArticleJournal of visualized experiments : JoVE2017
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Review
- Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale.Annual review of physical chemistry · 2021Review
- TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages.Scientific reports · 2021Article
- High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis.Nano letters · 2019Article
- Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data.Scientific reports · 2018Article
- Article
- Correlative imaging of biological tissues with apertureless scanning near-field optical microscopy and confocal laser scanning microscopy.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of super-resolution light microscopes allow improving the accuracy of the correlated data. Briefly, 110 nanometer thick cryo-sections are transferred to a silicon wafer and, after immunofluorescence staining, are imaged by super-resolution light microscopy. Subsequently, the sections are preserved in methylcellulose and platinum shadowed prior to imaging in a scanning electron microscope (SEM). The images from these two microscopy modalities are easily merged using tissue landmarks with open source software. Here we describe the adapted method for the larval zebrafish retina. However, this method is also applicable to other types of tissues and organisms. We demonstrate that the complementary information obtained by this correlation is able to resolve the expression of mitochondrial proteins in relation with the membranes and cristae of mitochondria as well as to other compartments of the cell.
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Registered trials
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