Evidence map›Paper›PMID 29155730›Full record

ArticleJournal of visualized experiments : JoVE2017

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae.

Kayla Huemer, Jayne M Squirrell, Robert Swader, Kirsten Pelkey, Danny C LeBert, Anna Huttenlocher, Kevin W Eliceiri

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. The Zebrafish as a Model Host for Invasive Fungal Infections.Journal of fungi (Basel, Switzerland) · 2018
    Review
  9. Stereolithographic 3D Printing with Renewable Acrylates.Journal of visualized experiments : JoVE · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kayla HuemerDepartment of Biomedical Engineering, University of Wisconsin-Madison; Morgridge Institute for Research, University of Wisconsin-Madison.
Jayne M SquirrellLaboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison.
Robert SwaderMorgridge Institute for Research, University of Wisconsin-Madison.
Kirsten PelkeyMorgridge Institute for Research, University of Wisconsin-Madison.
Danny C LeBertCellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison.
Anna HuttenlocherDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison; Department of Pediatrics, University of Wisconsin-Madison.
Kevin W EliceiriDepartment of Biomedical Engineering, University of Wisconsin-Madison; Morgridge Institute for Research, University of Wisconsin-Madison; Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison; eliceiri@wisc.edu.
University of Wisconsin–Madison · USOptica · USMorgridge Institute for Research · US

Funding

A toolkit for imaging and photo-manipulation of signaling in zebrafishR01GM102924 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HAHN, KLAUS M., HUTTENLOCHER, ANNA · 2012 to 2015
$2.5M
NIGMS NIH HHS R01 GM102924
6 · The paper itself

Abstract

The zebrafish larva is an important model organism for both developmental biology and wound healing. Further, the zebrafish larva is a valuable system for live high-resolution microscopic imaging of dynamic biological phenomena in space and time with cellular resolution. However, the traditional method of agarose encapsulation for live imaging can impede larval development and tissue regrowth. Therefore, this manuscript describes the zWEDGI (zebrafish Wounding and Entrapment Device for Growth and Imaging), which was designed and fabricated as a functionally compartmentalized device to orient larvae for high-resolution microscopy while permitting caudal fin transection within the device and subsequent unrestrained tail development and re-growth. This device allows for wounding and long-term imaging while maintaining viability. Given that the zWEDGI mold is 3D printed, the customizability of its geometries make it easily modified for diverse zebrafish imaging applications. Furthermore, the zWEDGI offers numerous benefits, such as access to the larva during experimentation for wounding or for the application of reagents, paralleled orientation of multiple larvae for streamlined imaging, and reusability of the device.

Indexed as

AnimalsDevelopmental BiologyDiagnostic ImagingDisease Models, AnimalLarvaZebrafish

Identifiers

PMID29155730
PMCPMC5755259
OpenAlexW2766049167

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.