ArticleJournal of the American Chemical Society2017
Fluorogenic Targeting of Voltage-Sensitive Dyes to Neurons.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Chemical basis of fluorogenic substrate probe design: revisiting the past to discover the new in biochemistry.RSC chemical biology · 2026Review
- Directed Evolution of Enzymes for Bioorthogonal Chemistry Using Acid Chloride Proximity Labeling.ACS central science · 2026Article
- Fluorogenic rhodamine B derivatives that become brighter at neutral pH.Bioorganic & medicinal chemistry letters · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors.ACS chemical neuroscience · 2025Review
- A chemical-genetic approach to target voltage-sensitive fluorophores to mitochondria.Mitochondrial communications · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Mammalian Esterase Activity: Implications for Peptide Prodrugs.Biochemistry · 2024Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators.Bioelectricity · 2024Article
- Fluorogenic Reactions in Chemical Biology: Seeing Chemistry in Cells.Chemical & biomedical imaging · 2023Review
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice.RSC chemical biology · 2021Article
- The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.Nature chemical biology · 2021Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- A far-red hybrid voltage indicator enabled by bioorthogonal engineering of rhodopsin on live neurons.Nature chemistry · 2021Article
- Optical Spike Detection and Connectivity Analysis With a Far-Red Voltage-Sensitive Fluorophore Reveals Changes to Network Connectivity in Development and Disease.Frontiers in neuroscience · 2021Article
- Voltage Imaging inFrontiers in neuroscience · 2021Article
- Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2.International journal of molecular sciences · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We present a method to target voltage-sensitive fluorescent dyes to specified cells using an enzyme-catalyzed fluorogenic reaction on cell surfaces. The dye/enzyme hybrids are composed of a photoinduced electron transfer (PeT)-based fluorescent voltage indicator and a complementary enzyme expressed on the cell surface. Action of the exogenous enzyme on the dye results in fluorogenic activation of the dye, enabling fast voltage imaging in defined neurons with sensitivity surpassing those of purely genetically encoded approaches. We employ a bulky methylcyclopropylacetoxymethyl ether to diminish the fluorescence of a PeT-based voltage-sensitive dye, or VoltageFluor. The hydrolytically stable ether can be removed by the action of porcine liver esterase (PLE) to reveal the bright unmodified VoltageFluor. We established that the chemically modified VoltageFluor is a substrate for PLE in vitro and in live cells. When PLE is targeted to the external face of cell membranes, it controls the apparent staining of cells. The use of neuron-specific promoters can direct staining to mammalian neurons to provide clear detection of neuronal action potentials in single trials. All of the new VoltageFluors targeted by esterase expression (VF-EXs) report single spikes in cultured mammalian neurons. The best, VF-EX2, does so with a signal-to-noise ratio nearly double that of comparable genetically encoded voltage reporters. By targeting PLE to neurons, VF-EX2 can interrogate the neuromodulatory effects of serotonin in cultured hippocampal neurons. Taken together, our results show that a combination of synthetic chemistry and biochemistry enables bright and fast voltage imaging from genetically defined neurons in culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.