ArticleMarine biotechnology (New York, N.Y.)2018
Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain).
Article in Marine biotechnology (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- High-resolution chromosome-level genome of Scylla paramamosain provides molecular insights into adaptive evolution in crabs.BMC biology · 2024Article
- Article
- Effects of berberine hydrochloride on immune response in the crab Charybdis japonica.BMC genomics · 2022Article
- Article
- Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus).BMC genomics · 2022Article
- Identification of Sex-Related Genes from the Three-Spot Swimming CrabAnimals : an open access journal from MDPI · 2021Article
- Comparative transcriptomic analysis for identification of candidate sex-related genes and pathways in Crimson seabream (Parargyrops edita).Scientific reports · 2021Article
- Full Functional Sex Reversal Achieved Through Silencing ofFrontiers in endocrinology · 2021Article
- Evidence of Sex Differentiation Based on Morphological Traits During the Early Development Stage of Mud CrabFrontiers in veterinary science · 2021Article
- The long non-coding RNA TSLC8 inhibits colorectal cancer by stabilizingCell cycle (Georgetown, Tex.) · 2020Article
- Genome-Wide Characterization of Alternative Splicing Events and Their Responses to Cold Stress in Tilapia.Frontiers in genetics · 2020Article
- De novo gonad transcriptome analysis of the common littoral shrimp Palaemon serratus: novel insights into sex-related genes.BMC genomics · 2019Article
- The Single-molecule long-read sequencing of Scylla paramamosain.Scientific reports · 2019Article
- Gonadal microRNA Expression Profiles and Their Potential Role in Sex Differentiation and Gonadal Maturation of Mud Crab Scylla paramamosain.Marine biotechnology (New York, N.Y.) · 2019Article
- High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Frontiers in genetics · 2019Article
- Effect of fasting and subsequent refeeding on the transcriptional profiles of brain in juvenile Spinibarbus hollandi.PloS one · 2019Article
- Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development.Marine biotechnology (New York, N.Y.) · 2018Article
- Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus.Scientific data · 2018Article
- Comparative Transcriptome Analysis Reveals Growth-Related Genes in Juvenile Chinese Sea Cucumber, Russian Sea Cucumber, and Their Hybrids.Marine biotechnology (New York, N.Y.) · 2018Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The molecular mechanism underlying sex determination and gonadal differentiation of the mud crab (Scylla paramamosain) has received considerable attention, due to the remarkably biological and economic differences between sexes. However, sex-biased genes, especially non-coding genes, which account for these differences, remain elusive in this crustacean species. In this study, the first de novo gonad transcriptome sequencing was performed to identify both differentially expressed genes and long non-coding RNAs (lncRNAs) between male and female S. paramamosain by using Illumina Hiseq2500. A total of 79,282,758 and 79,854,234 reads were generated from ovarian and testicular cDNA libraries, respectively. After filtrating and de novo assembly, 262,688 unigenes were produced from both libraries. Of these unigenes, 41,125 were annotated with known protein sequences in public databases. Homologous genes involved in sex determination and gonadal development pathways (Sxl-Tra/Tra-2-Dsx/Fru, Wnt4, thyroid hormone synthesis pathway, etc.) were identified. Three hundred and sixteen differentially expressed unigenes were further identified between both transcriptomes. Meanwhile, a total of 233,078 putative lncRNAs were predicted. Of these lncRNAs, 147 were differentially expressed between sexes. qRT-PCR results showed that nine lncRNAs negatively regulated the expression of eight genes, suggesting a potential role in sex differentiation. These findings will provide fundamental resources for further investigation on sex differentiation and regulatory mechanism in crustaceans.
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Registered trials
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