Evidence map›Paper›PMID 29152538›Full record

ArticleBio-protocol2017

Isolation of Primary Human Skeletal Muscle Cells.

Janelle M Spinazzola, Emanuela Gussoni

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Observational
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  19. Review
  20. Lysine methyltransferase 2D regulates muscle fiber size and muscle cell differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Janelle M SpinazzolaDivision of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA.
Emanuela GussoniDivision of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA.
Boston Children's Hospital · US

Funding

Tetraspanin CD82 in muscle satellite cells quiescence and differentiationR01AR069582 · NIAMS · BOSTON CHILDREN'S HOSPITAL · PI GUSSONI, EMANUELA · 2017 to 2022
$1.9M
NIAMS NIH HHS R01 AR069582
6 · The paper itself

Abstract

Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent

Indexed as

Fluorescence-activated cell sorting (FACS)Myoblast isolationSkeletal muscleTissue dissociation

Identifiers

PMID29152538
PMCPMC5687092
OpenAlexW2766969002

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.