ArticleLight, science & applications2017
Wide-field three-photon excitation in biological samples.
Article in Light, science & applications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.Light, science & applications · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Advances and future trends in real-time precision optical control of chemical processes in live cells.Npj imaging · 2025Review
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- DEEP-squared: deep learning powered De-scattering with Excitation Patterning.Light, science & applications · 2023Article
- Three-photon excited fluorescence imaging in neuroscience: From principles to applications.Frontiers in neuroscience · 2023Review
- Revealing the Wonder of Natural Photonics by Nonlinear Optics.Biomimetics (Basel, Switzerland) · 2022Article
- Tailored Multi-Color Dispersive Wave Formation in Quasi-Phase-Matched Exposed Core Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Article
- Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022Article
- Advances in nonlinear optical microscopy techniques for in vivo and in vitro neuroimaging.Biophysical reviews · 2021Review
- De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media.Science advances · 2021Article
- Holographic Imaging and Stimulation of Neural Circuits.Advances in experimental medicine and biology · 2021Article
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Scanless two-photon excitation with temporal focusing.Nature methods · 2020Review
- Article
- Photobleaching Imprinting Enhanced Background Rejection in Line-Scanning Temporal Focusing Microscopy.Frontiers in chemistry · 2020Article
- Optical Tweezers Exploring Neuroscience.Frontiers in bioengineering and biotechnology · 2020Review
- Simultaneous label-free autofluorescence and multi-harmonic imaging revealsBiomedical optics express · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.