ArticleCellular and molecular bioengineering2017
The Effect of Low-Magnitude Low-Frequency Vibrations (LMLF) on Osteogenic Differentiation Potential of Human Adipose Derived Mesenchymal Stem Cells.
Article in Cellular and molecular bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- A spiral microfluidic device for rapid sorting, trapping, and long-term live imaging ofMicrosystems & nanoengineering · 2023Article
- Production enhancement of human adipose-derived mesenchymal stem cells by low-intensity ultrasound stimulation.Scientific reports · 2022Article
- Frequency-specific sensitivity of 3T3-L1 preadipocytes to low-intensity vibratory stimulus during adipogenesis.In vitro cellular & developmental biology. Animal · 2022Article
- Mechanotransduction regulates inflammation responses of epicardial adipocytes in cardiovascular diseases.Frontiers in endocrinology · 2022Review
- The waves that make the pattern: a review on acoustic manipulation in biomedical research.Materials today. Bio · 2021Review
- MicroRNA-503-3p affects osteogenic differentiation of human adipose-derived stem cells by regulation of Wnt2 and Wnt7b under cyclic strain.Stem cell research & therapy · 2020Article
- Vibration acceleration promotes endochondral formation during fracture healing through cellular chondrogenic differentiation.PloS one · 2020Article
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Authors and funding
5 authors.
Funding
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Abstract
introductionIn the current study, we investigated the effect of low magnitude, low frequency (LMLF) mechanical vibrations on the osteogenic differentiation potential of human adipose derived mesenchymal stem cells (hASC), taken from elderly patients.
methodsDuring 21 days in osteogenic culture medium, cells were periodically exposed to three different frequencies (25, 35 and 45 Hz) of continuous sinusoidal oscillation, using a vibration generator. We measured cell proliferation, cell morphology, calcium and phosphorus deposition using Almar Blue assay, fluorescence microscopy, scanning electron microscopy, and a EDX detector, respectively. Osteogenic differentiation was measured by assessing protein and mRNA levels. Osteogenesis was confirmed by detection of specific markers with alkaline phosphatase and enzyme-linked immunosorbent assays for: bone morphogenetic protein 2 (BMP-2), osteocalcin (OCL) and osteopontin (OPN).
resultsWe found that 25 Hz vibrations had the greatest impact on hASC morphology, ultrastructure, and proliferation. We observed the formation of osteocyte- and hydroxyapatite-like structures, an increased quantity of calcium and phosphorus deposits, and increased differentiation in the stimulated groups.
conclusionsOur findings suggest that LMLF vibrations could be used to enhance cell-based therapies for treatment of bone deficits, particularly in elderly patients, where the need is greatest.
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