ArticleBMC genetics2017
Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers.
Article in BMC genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 87 citations in OpenAlex.
- Start codon targeted (SCoT) based molecular diversity and population structure analysis in black turmeric (Curcuma caesia Roxb.).BMC plant biology · 2026Article
- Molecular insights into salinity tolerance in Thymus daenensis: Scot and ISSR marker discovery in polycross-derived genotypes.BMC plant biology · 2025Article
- Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers.Journal, genetic engineering & biotechnology · 2025Article
- Genetic diversity and population structure of cowpea mutant collection using SSR and ISSR molecular markers.Scientific reports · 2024Article
- Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (Frontiers in plant science · 2024Article
- Article
- Molecular phylogeny of plant pathogenic fungi based on start codon targeted (SCoT) polymorphism.Molecular biology reports · 2023Article
- Characterization ofFrontiers in plant science · 2023Article
- Genetic analysis of walnut cultivars from southwest China: Implications for germplasm improvement.Plant diversity · 2022Article
- Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers.Molecular biology reports · 2022Article
- DNA Fingerprinting and Genetic Relationships Similarities Among the Accessions/Species of Ocimum Using SCoT and ISSR Markers System.Molecular biotechnology · 2021Article
- Genetic relationships and diversity analysis in Turkish laurel (Laurus nobilis L.) germplasm using ISSR and SCoT markers.Molecular biology reports · 2021Article
- Development, Identification, and Application of a Germplasm Specific SCAR Marker forFrontiers in plant science · 2021Article
- Comparative assessment of ISSR, RAPD, and SCoT markers for genetic diversity in Clerodendrum species of North East India.Molecular biology reports · 2020Article
- Genetic relationships and diversity among populations ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Evaluation of genetic relationship among varieties ofHeliyon · 2019Article
- Assessment of genetic diversity, population structure and sex identification in dioecious crop,Heliyon · 2019Article
- Genetic diversity and structure ofHereditas · 2019Article
- Biochemical and molecular characterization of cowpea landraces using seed storage proteins and SRAP marker patterns.Saudi journal of biological sciences · 2019Article
- Genetic diversity in the endangered terrestrial orchid Cypripedium japonicum in East Asia: Insights into population history and implications for conservation.Scientific reports · 2018Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAssessment of genetic diversity of Vigna unguiculata (L.) Walp (cowpea) accessions using informative molecular markers is imperative for their genetic improvement and conservation. Use of efficacious molecular markers to obtain the required knowledge of the genetic diversity within the local and regional germplasm collections can enhance the overall effectiveness of cowpea improvement programs, hence, the comparative assessment of Inter-simple sequence repeat (ISSR) and Start codon targeted (SCoT) markers in genetic diversity of V. unguiculata accessions from different regions in Nigeria. Comparative analysis of the genetic diversity of eighteen accessions from different locations in Nigeria was investigated using ISSR and SCoT markers. DNA extraction was done using Zymogen Kit according to its manufacturer's instructions followed by amplifications with ISSR and SCoT and agarose gel electrophoresis. The reproducible bands were scored for analyses of dendrograms, principal component analysis, genetic diversity, allele frequency, polymorphic information content, and population structure.
resultsBoth ISSR and SCoT markers resolved the accessions into five major clusters based on dendrogram and principal component analyses. Alleles of 32 and 52 were obtained with ISSR and SCoT, respectively. Numbers of alleles, gene diversity and polymorphic information content detected with ISSR were 9.4000, 0.7358 and 0.7192, while SCoT yielded 11.1667, 0.8158 and 0.8009, respectively. Polymorphic loci were 70 and 80 in ISSR and SCoT, respectively. Both markers produced high polymorphism (94.44-100%). The ranges of effective number of alleles (Ne) were 1.2887 ± 0.1797-1.7831 ± 0.2944 and 1.7416 ± 0.0776-1.9181 ± 0.2426 in ISSR and SCoT, respectively. The Nei's genetic diversity (H) ranged from 0.2112 ± 0.0600-0.4335 ± 0.1371 and 0.4111 ± 0.0226-0.4778 ± 0.1168 in ISSR and SCoT, respectively. Shannon's information index (I) from ISSR and SCoT were 0.3583 ± 0.0639-0.6237 ± 0.1759 and 0.5911 ± 0.0233-0.6706 ± 0.1604. Total gene diversity (Ht), gene diversity within population (Hs), coefficient of gene differentiation (Gst) and level of gene flow (Nm) revealed by ISSR were 0.4498, 0.3203, 0.2878 and 1.2371 respectively, while SCoT had 0.4808, 0.4522, 0.0594 and 7.9245.
conclusionsBoth markers showed highest genetic diversity in accessions from Ebonyi. Our study demonstrated that SCoT markers were more efficient than ISSR for genetic diversity studies in V. unguiculata and can be integrated in the exploration of their genetic diversity for improvement and germplasm utilization.
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