ArticlePlant molecular biology2018
SPL13 regulates shoot branching and flowering time in Medicago sativa.
Article in Plant molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 82 citations in OpenAlex.
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Article
- Identification and expression analysis of the SPL gene family during flower bud differentiation in Rhododendron molle.Genes & genomics · 2025Article
- Transcriptome Analysis Reveals Sugar and Hormone Signaling Pathways Mediating Flower Induction in Pitaya (International journal of molecular sciences · 2025Article
- Delineation of loci governing an extra-earliness trait in lentil (Lens culinaris Medik.) using the QTL-Seq approach.Plant biotechnology journal · 2024Article
- MsTFL1A delays flowering and regulates shoot architecture and root development in Medicago sativa.Plant reproduction · 2024Article
- Comprehensive Evolutionary Analysis of theInternational journal of molecular sciences · 2024Article
- Genome-wide investigation ofAoB PLANTS · 2024Article
- Overexpression of lily MicroRNA156-resistantFrontiers in plant science · 2024Article
- Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in citrus relatives with a specific focus on flowering.Molecular horticulture · 2023Article
- Oak stands along an elevation gradient have different molecular strategies for regulating bud phenology.BMC plant biology · 2023Article
- Molecular characterization of SPL gene family during flower morphogenesis and regulation in blueberry.BMC plant biology · 2023Article
- Article
- Jasmonic acid negatively regulates branch growth in pear.Frontiers in plant science · 2023Article
- Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa.Plant molecular biology · 2022Article
- Integrating ATAC-seq and RNA-seq Reveals the Dynamics of Chromatin Accessibility and Gene Expression in Apple Response to Drought.International journal of molecular sciences · 2022Article
- The Role of Transcription Factors in the Regulation of Plant Shoot Branching.Plants (Basel, Switzerland) · 2022Review
- Leaf Development inGenes · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
key messageOur results show SPL13 plays a crucial role in regulating vegetative and reproductive development in Medicago sativa L. (alfalfa), and that MYB112 is targeted and downregulated by SPL13 in alfalfa. We previously showed that transgenic Medicago sativa (alfalfa) plants overexpressing microRNA156 (miR156) show a bushy phenotype, reduced internodal length, delayed flowering time, and enhanced biomass yield. In alfalfa, transcripts of seven SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, including SPL13, are targeted for cleavage by miR156. Thus, association of each target SPL gene to a trait or set of traits is essential for developing molecular markers for alfalfa breeding. In this study, we investigated SPL13 function using SPL13 overexpression and silenced alfalfa plants. Severe growth retardation, distorted branches and up-curled leaves were observed in miR156-impervious 35S::SPL13m over-expression plants. In contrast, more lateral branches and delayed flowering time were observed in SPL13 silenced plants. SPL13 transcripts were predominantly present in the plant meristems, indicating that SPL13 is involved in regulating shoot branch development. Accordingly, the shoot branching-related CAROTENOID CLEAVAGE DIOXYGENASE 8 gene was found to be significantly downregulated in SPL13 RNAi silencing plants. A R2R3-MYB gene MYB112 was also identified as being directly silenced by SPL13 based on Next Generation Sequencing-mediated transcriptome analysis and chromatin immunoprecipitation assays, suggesting that MYB112 may be involved in regulating alfalfa vegetative growth.
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Registered trials
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