ArticleMethods and applications in fluorescence2013
Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR.
Article in Methods and applications in fluorescence, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- PolSpec : Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging.Journal of biophotonics · 2025Article
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- HyU: Hybrid Unmixing for longitudinal in vivo imaging of low signal-to-noise fluorescence.Nature methods · 2023Article
- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Full-Harmonics Phasor Analysis: Unravelling Multiexponential Trends in Magnetic Resonance Imaging Data.The journal of physical chemistry letters · 2020Article
- Pre-processing visualization of hyperspectral fluorescent data with Spectrally Encoded Enhanced Representations.Nature communications · 2020Article
- Hyperspectral imaging in highly scattering media by the spectral phasor approach using two filters.Biomedical optics express · 2018Article
- Multispectral analysis tools can increase utility of RGB color images in histology.Journal of optics (2010) · 2018Article
- Application of Spectral Phasor analysis to sodium microenvironments in myoblast progenitor cells.PloS one · 2018Article
- Phasor plotting with frequency-domain flow cytometry.Optics express · 2016Article
- Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method.Microscopy research and technique · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.
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Registered trials
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