ArticleEnvironmental epigenetics2017
Generational comparisons (F1 versus F3) of vinclozolin induced epigenetic transgenerational inheritance of sperm differential DNA methylation regions (epimutations) using MeDIP-Seq.
Article in Environmental epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Transgenerational epigenetic effects from male exposure to endocrine-disrupting compounds: a systematic review on research in mammals.Clinical epigenetics · 2020Pooled it
- Article
- Stability of epigenetic transgenerational inheritance of adult-onset disease and parturition abnormalities.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Bridging the Gap: How environmental exposures compromise male fertility and clinical Outlook.Current research in toxicology · 2026Review
- From Lineage to Longevity: A Field Guide to the Key Players in Epigenetic Contribution to Offspring Health.Current issues in molecular biology · 2025Review
- Generational stability of environmentally induced epigenetic transgenerational inheritance of adult-onset disease over ten mammalian generations.Environmental epigenetics · 2025Article
- Generational stability of epigenetic transgenerational inheritance facilitates adaptation and evolution.Epigenetics · 2024Review
- Epigenetics Research in Evolutionary Biology: Perspectives on Timescales and Mechanisms.Molecular biology and evolution · 2024Review
- Cold exposure impacts DNA methylation patterns in cattle sperm.Frontiers in genetics · 2024Article
- Epigenetic Inheritance and Transgenerational Environmental Justice.The Yale journal of biology and medicine · 2023Review
- Multi- and Transgenerational Effects of Environmental Toxicants on Mammalian Reproduction.Cells · 2022Review
- Article
- Impact of Endocrine Disruptors upon Non-Genetic Inheritance.International journal of molecular sciences · 2022Review
- Environmental factors in declining human fertility.Nature reviews. Endocrinology · 2022Review
- Developmental alterations in DNA methylation during gametogenesis from primordial germ cells to sperm.iScience · 2022Article
- Paternal transmission of behavioural and metabolic traits induced by postnatal stress to the 5th generation in mice.Environmental epigenetics · 2022Article
- Epigenetic transgenerational inheritance, gametogenesis and germline development†.Biology of reproduction · 2021Review
- Defective folate metabolism causes germline epigenetic instability and distinguishes Hira as a phenotype inheritance biomarker.Nature communications · 2021Article
- Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Endocrine disrupting chemicals in the pathogenesis of hypospadias; developmental and toxicological perspectives.Current research in toxicology · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Environmentally induced epigenetic transgenerational inheritance of disease and phenotypic variation has been shown to involve DNA methylation alterations in the germline (e.g. sperm). These differential DNA methylation regions (DMRs) are termed epimutations and in part transmit the transgenerational phenotypes. The agricultural fungicide vinclozolin exposure of a gestating female rat has previously been shown to promote transgenerational disease and epimutations in F3 generation (great-grand-offspring) animals. The current study was designed to investigate the actions of direct fetal exposure on the F1 generation rat sperm DMRs compared to the F3 transgenerational sperm DMRs. A protocol involving methylated DNA immunoprecipitation (MeDIP) followed by next-generation sequencing (Seq) was used in the current study. Bioinformatics analysis of the MeDIP-Seq data was developed and several different variations in the bioinformatic analysis were evaluated. Observations indicate needs to be considered. Interestingly, the F1 generation DMRs were found to be fewer in number and for the most part distinct from the F3 generation epimutations. Observations suggest the direct exposure induced F1 generation sperm DMRs appear to promote in subsequent generations alterations in the germ cell developmental programming that leads to the distinct epimutations in the F3 generation. This may help explain the differences in disease and phenotypes between the direct exposure F1 generation and transgenerational F3 generation. Observations demonstrate a distinction between the direct exposure versus transgenerational epigenetic programming induced by environmental exposures and provide insights into the molecular mechanisms involved in the epigenetic transgenerational inheritance phenomenon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.