ArticleScientific reports2017
Real-time wash-free detection of unlabeled PNA-DNA hybridization using discrete FET sensor.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 36 citations in OpenAlex.
- Ionic Strength Investigation on the Interaction Between miR-155 and a PNA-Based Probe by Atomic Force Spectroscopy.Biomolecules · 2025Article
- Recent advances in methods for quantifying the cell penetration of macromolecules.Current opinion in chemical biology · 2024Review
- Detection of miR-155 Using Peptide Nucleic Acid at Physiological-like Conditions by Surface Plasmon Resonance and Bio-Field Effect Transistor.Biosensors · 2024Article
- Hybridization Kinetics of miR-155 on Gold Surfaces as Investigated by Surface Plasmon Resonance and Atomic Force Spectroscopy.ACS omega · 2023Article
- Integrated Approach from Sample-to-Answer for Grapevine Varietal Identification on a Portable Graphene Sensor Chip.ACS sensors · 2023Article
- Biofunctionalization of Multiplexed Silicon Photonic Biosensors.Biosensors · 2022Review
- Nucleic Acid Identity, Structure, and Flexibility Affect the Electrochemical Signal of Tethered Redox Molecules upon Biopolymer Collapse.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Bio-Separated and Gate-Free 2D MoSNanomaterials (Basel, Switzerland) · 2021Article
- Biosensing based on field-effect transistors (FET): Recent progress and challenges.Trends in analytical chemistry : TRAC · 2020Review
- A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment.Sensors (Basel, Switzerland) · 2020Article
- Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors.Nano letters · 2020Article
- Article
- Magnetic Graphene Field-Effect Transistor Biosensor for Single-Strand DNA Detection.Nanoscale research letters · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA backbone. Similar detection schemes have mainly concentrated on sensitivity improvement with an emphasis on new sensor structures. Our approach focuses on using an extended-gate that separates the FET and the sensing electrode yielding a simple and mass fabricable device. We used PNA probes for efficient hybridization in low salt conditions that is required to avoid the counter ion screening. As a result, significant part of the target DNA lies within the screening length of the sensor. With this, we achieved a wash-free detection where typical gate potential shifts are more than 70 mV with 1 µM target DNA. We routinely obtained a real-time, label- and wash-free specific detection of target DNA in nanomolar concentration with low-cost electronics and the responses were achieved within minutes after introducing targets to the solution. Furthermore, the results suggest that the sensor performance is limited by specificity rather than by sensitivity and using low-cost electronics does not limit the sensor performance in the presented sensor configuration.
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