ArticleScientific reports2017
Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0-1 and used for biodiesel production.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Microbial lipases: advances in metagenomics and artificial intelligence for enzyme discovery and engineering.Archives of microbiology · 2026Review
- Metal-Dependent Effects and Crowding Robustness of Pseudomonas fluorescens Esterase I.Chembiochem : a European journal of chemical biology · 2026Article
- Heterologous expression and foldase-assisted refolding of LipGoM, aBiochemistry and biophysics reports · 2025Article
- Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.Microbial cell factories · 2025Review
- Recent insight into the advances and prospects of microbial lipases and their potential applications in industry.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Review
- Molecular identification of Halomonas AZ07 and its multifunctional enzymatic activities to degrade Pyropia yezoensis under high-temperature condition.Molecular biology reports · 2024Article
- Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization.PloS one · 2024Article
- Characterization of Detergent-Compatible Lipases fromACS omega · 2023Article
- The Recent Advances in the Utility of Microbial Lipases: A Review.Microorganisms · 2023Review
- Intracellular-to-extracellular localization switch of acidic lipase in Enterobacter cloacae through multi-objective medium optimization: aqueous two-phase purification and activity kinetics.World journal of microbiology & biotechnology · 2022Article
- Optimization of lipase production using fungal isolates from oily residues.BMC biotechnology · 2021Article
- Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea.Antonie van Leeuwenhoek · 2021Article
- Response of particle-associated bacteria to long-term heavy metal contamination in a tropical estuary.World journal of microbiology & biotechnology · 2020Article
- The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena.Microbial biotechnology · 2020Review
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3 authors.
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Abstract
As a kind of important biocatalysts, Pseudomonas lipases are commonly applied in various industrial fields. Pflip1, a new extracellular lipase gene from Pseudomonas. fluorescens Pf0-1, was first cloned and respectively expressed in Escherichia coli BL21(DE3) and Pichia pastoris KM71, the recombinant proteins Pflip1a and Pflip1b were later purified separately. Then Pflip1a was further characterized. The optimum pH of Pflip1a was 8.0 and its optimal temperature was 70 °C. After incubation at 70 °C for 12 h, Pflip1a could retain over 95% of its original activity. It showed the highest activity towards p-nitrophenyl caprylate. Moreover, its activity was profoundly affected by metal ion, ionic surfactants and organic solvents. Furthermore, the two obtained recombinant lipases were immobilized on the magnetic nanoparticles for biodiesel preparation. The GC analysis showed that for the immobilized lipases Pflip1b and Pflip1a, the biodiesel yield within 24 h respectively attained 68.5% and 80.5% at 70 °C. The activities of the two immobilized lipases still remained 70% and 82% after 10 cycles of operations in non-solvent system. These characteristics and transesterification capacity of the recombinant protein indicated its great potential for organic synthesis, especially for biodiesel production.
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