ArticlePloS one2017
Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio).
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 68 citations in OpenAlex.
- dUTPase is essential in zebrafish development and possesses several single-nucleotide variants with pronounced structural and functional consequences.FEBS open bio · 2026Article
- An Overview of Advancements in Proteomic Approaches to Enhance Livestock Production and Aquaculture.Animals : an open access journal from MDPI · 2025Review
- Binding of zebrafish lipovitellin and L1‑ORF2 increases the accessibility of L1‑ORF2 via interference with histone wrapping.International journal of molecular medicine · 2025Article
- Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis.Cell reports · 2024Article
- Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon.BMC genomics · 2024Article
- Evolutionarily conserved ovarian fluid proteins are responsible for extending egg viability in salmonid fish.Scientific reports · 2024Article
- The constructive and destructive impact of autophagy on both genders' reproducibility, a comprehensive review.Autophagy · 2023Review
- Genomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio).Scientific reports · 2023Article
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- The effects of intensive feeding on reproductive performance in laboratory zebrafish (Danio rerio).PloS one · 2022Article
- Probiotic Administration Mitigates Bisphenol A Reproductive Toxicity in Zebrafish.International journal of molecular sciences · 2021Article
- Knock out of specific maternal vitellogenins in zebrafish (Danio rerio) evokes vital changes in egg proteomic profiles that resemble the phenotype of poor quality eggs.BMC genomics · 2021Article
- Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns.Scientific reports · 2021Article
- Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition.PLoS genetics · 2021Article
- Liquid Chromatography and Tandem Mass Spectrometry in Label-Free Protein Quantification of Zebrafish (Danio rerio) Eggs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- About Welfare and Stress in the Early Stages of Fish.Frontiers in veterinary science · 2021Article
- Knockout of the Glucocorticoid Receptor Impairs Reproduction in Female Zebrafish.International journal of molecular sciences · 2020Article
- What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization.BMC genomics · 2019Article
- Multiple vitellogenins in zebrafish (Danio rerio): quantitative inventory of genes, transcripts and proteins, and relation to egg quality.Fish physiology and biochemistry · 2018Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry. Obtained spectra were searched against a zebrafish proteome database and detected proteins were annotated, categorized and quantified based on normalized spectral counts. Manually curated and automated enrichment analyses revealed poor quality eggs to be deficient of proteins involved in protein synthesis and energy and lipid metabolism, and of some vitellogenin products and lectins, and to have a surfeit of proteins involved in endo-lysosomal activities, autophagy, and apoptosis, and of some oncogene products, lectins and egg envelope proteins. Results of pathway and network analyses suggest that this aberrant proteomic profile results from failure of oocytes giving rise to poor quality eggs to properly transit through final maturation, and implicated Wnt signaling in the etiology of this defect. Quantitative comparisons of abundant proteins in good versus poor quality eggs revealed 17 candidate egg quality markers. Thus, the zebrafish egg proteome is clearly linked to embryo developmental potential, a phenomenon that begs further investigation to elucidate the root causes of poor egg quality, presently a serious and intractable problem in livestock and human reproductive medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.