Evidence map›Paper›PMID 29143185›Full record

ArticleJournal of materials science. Materials in medicine2017

Osteogenic prospective of deriving human dental stem cells in collagen matrix boost.

Tong-Jing Fang, Ding-Han Wang, Chia-Yu Wang, Raju Poongodi, Nien-Hsien Liou, Jiang-Chuan Liu, Ming-Lun Hsu, Po-Da Hong, Shih-Fang Yang, Meng-Lun Liu

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In one paragraph

Article in Journal of materials science. Materials in medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Tong-Jing FangGraduate Institute of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, China.
Ding-Han WangSchool of Dentistry, National Yang-Ming University, Taipei, Taiwan, China.
Chia-Yu WangGraduate Institute of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, China.
Raju PoongodiLab of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, Taipei, Taiwan, China.
Nien-Hsien LiouDepartment of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, China.
Jiang-Chuan LiuDepartment of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, China.
Ming-Lun HsuSchool of Dentistry, National Yang-Ming University, Taipei, Taiwan, China.
Po-Da HongGraduate Institute of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, China.
Shih-Fang YangSchool of Dentistry, National Yang-Ming University, Taipei, Taiwan, China. sfyang2017@gmail.com.
Meng-Lun LiuDepartment of Surgery, Cheng Hsin General Hospital, Taipei, Taiwan, China. doc20054@gmail.com.

Funding

Cheng Hsin General Hospital and National Yan-Ming University cooperation grant 101F195C15Cheng Hsin General Hospital and National Yan-Ming University cooperation grant 98F117CY21
6 · The paper itself

Abstract

Stem cells derived from oral tissue represent a highly attractive alternative source for clinical bone regeneration because they can be collected by non-invasive or minimally invasive procedures. Herein, we describe the human dental stem cells (DSCs) deriving from buccal fat pads (BFP), dental pulp (DP) of impacted teeth, and periodontal ligaments (PDL) to obtain BFPSCs, DPSCs, and PDLSCs, respectively. Cells were purified with selected medium and expanded through passages in stem cell culture medium. Purified cells were characterized for stemness by their growth rate, immunostaining, and multilineage differentiation ability. They showed plastic adherence, expression of stemness-specific markers, and multilineage differentiation potential. Immunocytochemistry analysis confirmed that DPSCs had more osteogenic potential than BFSCs and PDLSCs. Calcium-rich deposits, evaluated by von Kossa and Alizarin red staining, showed greater mineralization when DPSCs were cultured on collagen type I matrix than without collagen. Furthermore, DPSC-seeded collagen type I matrix maintained consistent osteogenesis and boosted mineral formation by 1-2 weeks over that in DPSCs cultured without collagen. Radiographic analysis of DPSC-seeded collagen type I matrix transplanted into rat cranial defects showed significant bone regeneration after 8 weeks. These results suggested that the redundant oral tissue can be used as a source of adult multipotent stem cells for clinical bone regeneration. Triple overlay images with biomarkers (red), nuclei (blue) and bright field morphology of DPSCs. The specifically osteo-differentiation shown by osteocalcin (left) expression and lack of sox9 (right) expressed in the images below which were cultured with collagen matrix, contrast with no collagen matrix group above.

Indexed as

Collagen Type IAdipose TissueAnimalsBone TransplantationCell Culture TechniquesDental PulpHumansOsteogenesisPeriodontal LigamentRatsStem CellsCollagen Type I

Identifiers

PMID29143185

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.