ArticleMetabolomics : Official journal of the Metabolomic Society2017
Using an NMR metabolomics approach to investigate the pathogenicity of amyloid-beta and alpha-synuclein.
Article in Metabolomics : Official journal of the Metabolomic Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Metabolic Reprogramming Associated with Ferroptosis Protection by an Indole-Based Antioxidant in Aβ(25-35)-Treated SH-SY5Y Cells.Antioxidants (Basel, Switzerland) · 2026Article
- Analyzing Nicotine Action Against Amyloid Toxicity by NMR-Pharmacometabolomics: An Exploratory Study.NMR in biomedicine · 2026Article
- Article
- Novel metabolic biomarker for early detection and diagnosis to the patients with gastric cardia adenocarcinoma.Cancer medicine · 2024Article
- Chemical catalysis by biological amyloids.Biochemical Society transactions · 2023Article
- Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites.International journal of molecular sciences · 2023Article
- Amyloid Fibers of α-Synuclein Catalyze Chemical Reactions.ACS chemical neuroscience · 2023Article
- New Evidence on a Distinction between Aβ40 and Aβ42 Amyloids: Thioflavin T Binding Modes, Clustering Tendency, Degradation Resistance, and Cross-Seeding.International journal of molecular sciences · 2022Article
- High Resolution Magic Angle Spinning Proton NMR Study of Alzheimer's Disease with Mouse Models.Metabolites · 2022Article
- Metabolite and lipoprotein profiles reveal sex-related oxidative stress imbalance in de novo drug-naive Parkinson's disease patients.NPJ Parkinson's disease · 2022Article
- Article
- The Diagnostic Potential of Amyloidogenic Proteins.International journal of molecular sciences · 2021Review
- Article
- Recent advances and perspectives of metabolomics-based investigations in Parkinson's disease.Molecular neurodegeneration · 2019Review
- A new era for understanding amyloid structures and disease.Nature reviews. Molecular cell biology · 2018Review
- A robust intracellular metabolite extraction protocol for human neutrophil metabolic profiling.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
introductionThe pathogenicity at differing points along the aggregation pathway of many fibril-forming proteins associated with neurodegenerative diseases is unclear. Understanding the effect of different aggregation states of these proteins on cellular processes is essential to enhance understanding of diseases and provide future options for diagnosis and therapeutic intervention.
objectivesTo establish a robust method to probe the metabolic changes of neuronal cells and use it to monitor cellular response to challenge with three amyloidogenic proteins associated with neurodegenerative diseases in different aggregation states.
methodNeuroblastoma SH-SY5Y cells were employed to design a robust routine system to perform a statistically rigorous NMR metabolomics study into cellular effects of sub-toxic levels of alpha-synuclein, amyloid-beta 40 and amyloid-beta 42 in monomeric, oligomeric and fibrillar conformations.
resultsThis investigation developed a rigorous model to monitor intracellular metabolic profiles of neuronal cells through combination of existing methods. This model revealed eight key metabolites that are altered when neuroblastoma cells are challenged with proteins in different aggregation states. Metabolic pathways associated with lipid metabolism, neurotransmission and adaptation to oxidative stress and inflammation are the predominant contributors to the cellular variance and intracellular metabolite levels. The observed metabolite changes for monomer and oligomer challenge may represent cellular effort to counteract the pathogenicity of the challenge, whereas fibrillar challenge is indicative of system shutdown. This implies that although markers of stress are more prevalent under oligomeric challenge the fibrillar response suggests a more toxic environment.
conclusionThis approach is applicable to any cell type that can be cultured in a laboratory (primary or cell line) as a method of investigating how protein challenge affects signalling pathways, providing additional understanding as to the role of protein aggregation in neurodegenerative disease initiation and progression.
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