ArticleClinical proteomics2017
Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis.
Article in Clinical proteomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Diagnosis of tuberculosis in wildlife: a systematic review.Veterinary research · 2021Pooled it
- Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis.Nature protocols · 2026Review
- Livestock immunomodulators as alternatives to antimicrobials - a roadmap for the fight against drug resistance.BMC veterinary research · 2026Review
- Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection ofJournal of clinical microbiology · 2026Article
- Exploring alternative cytokines as potential biomarkers forFrontiers in immunology · 2026Article
- Protective effect of heat-inactivated Mycobacterium bovis applied intramuscularly is associated with enhanced lung immune response in caprine tuberculosis.Veterinary research · 2025Article
- Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area.Frontiers in veterinary science · 2025Article
- Protection and diagnostic interference induced by heat-inactivated, phage-inactivated and live vaccine prototypes against animal tuberculosis.Frontiers in veterinary science · 2025Article
- Evaluation of serological assays forFrontiers in microbiology · 2025Article
- A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats.The veterinary quarterly · 2024Article
- Genomic comparison betweenFrontiers in veterinary science · 2024Article
- Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis.Frontiers in veterinary science · 2024Article
- Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds.Frontiers in veterinary science · 2024Article
- Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria.Scientific reports · 2023Article
- Comparison of the pathological outcome and disease progression of twoFrontiers in microbiology · 2023Article
- Article
- A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats.Scientific reports · 2022Article
- Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs.Veterinary research · 2022Article
- Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats.BMC veterinary research · 2022Article
- Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between
methodsTrypsin digests of bPPD, aPPD and P22 were analysed by nanoscale liquid chromatography-electrospray ionization tandem mass spectrometry. Mice were immunised with bPPD or aPPD, and their serum was tested by indirect ELISA for reactivity against these preparations as well as against P22.
resultsA total of 456 proteins were identified in bPPD, 1019 in aPPD and 118 in P22; 146 of these proteins were shared by bPPD and aPPD, and 43 were present in all three preparations. Candidate proteins that may cause cross-reactivity between bPPD and aPPD were identified based on protein abundance and antigenic propensity. Serum reactivity experiments indicated that P22 may provide greater specificity than bPPD with similar sensitivity for ELISA-type detection of antibodies against
conclusionThe subpreparation from bPPD called P22 may be an alternative to bPPD for serodiagnosis of bovine tuberculosis, since it shares fewer proteins with aPPD than bPPD does, reducing risk of cross-reactivity with anti-
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