ArticleScientific reports2017
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
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- Feedback-Driven Dynamical Model for Axonal Extension on Parallel Micropatterns.Biomimetics (Basel, Switzerland) · 2025Article
- Roll-to-Roll (R2R) High-Throughput Manufacturing of Foil-Based Microfluidic Chips for Neurite Outgrowth Studies.Micromachines · 2025Article
- A high-fidelity microfluidic platform reveals retrograde propagation as the main mechanism of α-Synuclein spread in human neurons.NPJ Parkinson's disease · 2025Article
- Open-End Control of Neurite Outgrowth Lengths with Steep Bending Confinement Microchannel Patterns for Miswiring-Free Neuronal Network Formation.Micromachines · 2024Article
- Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.Nano letters · 2024Article
- Reverse engineering of feedforward cortical-Hippocampal microcircuits for modelling neural network function and dysfunction.Scientific reports · 2024Article
- Exploring Kainic Acid-Induced Alterations in Circular Tripartite Networks with Advanced Analysis Tools.eNeuro · 2024Article
- The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro.Micromachines · 2024Article
- Nonlinear Growth Dynamics of Neuronal Cells Cultured on Directional Surfaces.Biomimetics (Basel, Switzerland) · 2024Article
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Mind In Vitro Platforms: Versatile, Scalable, Robust, and Open Solutions to Interfacing with Living Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A flexible, thin-film microchannel electrode array device for selective subdiaphragmatic vagus nerve recording.Microsystems & nanoengineering · 2024Article
- Connectivity and network burst properties of in-vitro neuronal networks induced by a clustered structure with alginate hydrogel patterning.Biomedical engineering letters · 2023Article
- Multiple neuron clusters on Micro-Electrode Arrays as an in vitro model of brain network.Scientific reports · 2023Article
- Toward the Next Generation of Neural Iontronic Interfaces.Advanced healthcare materials · 2023Article
- Microfluidic Bi-Layer Platform to Study Functional Interaction between Co-Cultured Neural Networks with Unidirectional Synaptic Connectivity.Micromachines · 2023Article
- Recent Progress and Perspectives on Neural Chip Platforms Integrating PDMS-Based Microfluidic Devices and Microelectrode Arrays.Micromachines · 2023Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The architecture of neuron connectivity in brain networks is one of the basic mechanisms by which to organize and sustain a particular function of the brain circuitry. There are areas of the brain composed of well-organized layers of neurons connected by unidirectional synaptic connections (e.g., cortex, hippocampus). Re-engineering of the neural circuits with such a heterogeneous network structure in culture may uncover basic mechanisms of emergent information functions of these circuits. In this study, we present such a model designed with two subpopulations of primary hippocampal neurons (E18) with directed connectivity grown in a microfluidic device with asymmetric channels. We analysed and compared neurite growth in the microchannels with various shapes that promoted growth dominantly in one direction. We found an optimal geometric shape features of the microchannels in which the axons coupled two chambers with the neurons. The axons grew in the promoted direction and formed predefined connections during the first 6 days in vitro (DIV). The microfluidic devices were coupled with microelectrode arrays (MEAs) to confirm unidirectional spiking pattern propagation through the microchannels between two compartments. We found that, during culture development, the defined morphological and functional connectivity formed and was maintained for up to 25 DIV.
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