ArticlePloS one2017
Development, standardization and testing of a bacterial wound infection model based on ex vivo human skin.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Evaluation of bacteriophage efficacy against Pseudomonas aeruginosa in ex vivo and in vitro canine skin systems.Scientific reports · 2026Article
- Diagnostic electron microscopy in human infectious diseases - Methods and applications.Journal of microscopy · 2025Review
- Octenidine effectively reduces Candida auris colonisation on human skin.Scientific reports · 2025Article
- An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections.Advanced healthcare materials · 2024Article
- Secondary metabolites in topical infectious diseases and nanomedicine applications.Nanomedicine (London, England) · 2024Review
- Article
- Ciprofloxacin-Loaded Polyvinylpyrrolidone Foils for the Topical Treatment of Wound Infections with Methicillin-ResistantPharmaceutics · 2023Article
- Article
- Effects of Adipose-Derived Stem Cells and Their Conditioned Medium in a Human Ex Vivo Wound Model.Cells · 2022Article
- Systems of conductive skin for power transfer in clinical applications.European biophysics journal : EBJ · 2022Review
- Evaluation of a Multifunctional Polyvinylpyrrolidone/Hyaluronic Acid-Based Bilayer Film Patch with Anti-Inflammatory Properties as an Enhancer of the Wound Healing Process.Pharmaceutics · 2022Article
- Nanocarriers for Skin Applications: Where Do We Stand?Angewandte Chemie (International ed. in English) · 2022Review
- Screening of Surfactants for Improved Delivery of Antimicrobials and Poly-Lactic-Pharmaceutics · 2021Article
- Vancomycin-Loaded Microneedle Arrays against Methicillin-ResistantAdvanced materials technologies · 2021Article
- An ex vivo model of medical device-mediated bacterial skin translocation.Scientific reports · 2021Article
- Comparison of Two Hair Removal Methods in Sprague-Dawley Rats (Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Polyphosphazenes enable durable, hemocompatible, highly efficient antibacterial coatings.Biomaterials · 2021Article
- Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior in Simulated Human Body Fluids and Effect on Macrophages.Polymers · 2020Article
- Article
- Manuka honey microneedles for enhanced wound healing and the prevention and/or treatment of Methicillin-resistant Staphylococcus aureus (MRSA) surgical site infection.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using-otherwise discarded-skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis. At the same time, IL-1alpha and IL-1beta amounts increased in all infected wounds, while-due to bacteria-induced cell lysis-the IL-6 and IL-8 concentrations rose only in the uninfected samples. High-dosage ciprofloxacin treatment resulted in a decisive decrease in bacteria, but consistently failed to eradicate all bacteria. The main benefits of the ex vivo wound model are the use of healthy human skin, a quantifiable bacterial infection, a measureable donor-dependent immune response and a good repeatability of the results. These properties turn the ex vivo wound model into a valuable tool to examine the mechanisms of host-pathogen interactions and to test antimicrobial agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.