ArticleInternational journal of immunopathology and pharmacology2017
Conditioned medium from human gingival mesenchymal stem cells protects motor-neuron-like NSC-34 cells against scratch-injury-induced cell death.
Article in International journal of immunopathology and pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 50 citations in OpenAlex.
- Propolis nanoemulsion and mesenchymal stem cell conditioned medium promote osteoblastogenesis against lipopolysaccharide-induced osteolysis in hyperglycemic rats.Scientific reports · 2026Article
- Advanced lyophilized mesenchymal stem cell-conditioned medium formulation improves therapeutic efficacy in skin wound repair.Regenerative biomaterials · 2026Article
- MiRNAs profile of extracellular vesicles from differentiated endothelial cells treated with chemical environmental pollutant BPA.Scientific reports · 2025Article
- Effect of Osteogenic Metabolic Differentiation of Silver Nanoparticles-based Periodontal Ligament Fibroblasts on Orthodontic Tooth Movement.Cell biochemistry and biophysics · 2025Article
- LINC00673 binds with leptin to regulate osteogenic function in periodontal ligament stem cells.Journal of orthopaedic surgery and research · 2025Article
- Adipose Tissue-Derived Exosomes as a Novel Therapeutic Approach for Refractory Alopecia Areata: A Report of Two Cases.Case reports in dermatological medicine · 2025Article
- Mechanical scratch injury on differentiated motor neuron of NSC-34 cells as an in vitro model for evaluation of neuroregeneration potential of NeuroAiD II (MLC901).In vitro models · 2024Article
- Biological Effects of PMMA and Composite Resins on Human Gingival Fibroblasts: An In Vitro Comparative Study.International journal of molecular sciences · 2024Article
- Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process.Frontiers in bioengineering and biotechnology · 2024Article
- Engineered extracellular vesicles for tissue repair and regeneration.Burns & trauma · 2024Review
- Analogies and Differences Between Dental Stem Cells: Focus on Secretome in Combination with Scaffolds in Neurological Disorders.Stem cell reviews and reports · 2024Review
- Therapeutic Potential of Oral-Derived Mesenchymal Stem Cells in Retinal Repair.Stem cell reviews and reports · 2023Review
- Transcriptomic Profiling after In Vitro ΔPharmaceuticals (Basel, Switzerland) · 2023Article
- Wharton's jelly mesenchymal stem cells: a concise review of their secretome and prospective clinical applications.Frontiers in cell and developmental biology · 2023Review
- The role of primed and non-primed MSC-derived conditioned media in neuroregeneration.Frontiers in molecular neuroscience · 2023Article
- Article
- Promising Role of Oral Cavity Mesenchymal Stem Cell-Derived Extracellular Vesicles in Neurodegenerative Diseases.Molecular neurobiology · 2022Review
- Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea.International journal of environmental research and public health · 2022Article
- Dental stem cell-conditioned medium for tissue regeneration: Optimization of production and storage.World journal of stem cells · 2022Article
- A Narrative Review: Gingival Stem Cells as a Limitless Reservoir for Regenerative Medicine.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuronal cell death is a normal process during central nervous system (CNS) development and is also involved in the death of motor neurons in diverse spinal motor neuron degenerative diseases. Here, we investigated the neuroprotective effect of secretory factors released from human gingival mesenchymal stem cells (hGMSCs) in mechanically injured murine motor-neuron-like NSC-34 cells. The cells were exposed to scratch injury and the markers for apoptosis and oxidative stress were examined. Immunocytochemistry results showed that proapoptotic markers cleaved caspase-3 and Bax were elevated while anti-apoptotic protein Bcl-2 was suppressed in scratch-injured NSC-34 cells. Oxidative stress markers SOD-1, inducible nitric oxide synthase (iNOS), Cox-2, and proinflammatory cytokine tumor necrosis factor alpha (TNF-α) were activated. Conditioned medium (CM) derived from hGMSCs (hGMSC-CM) significantly blocked the cell death by suppressing SOD-1, iNOS, TNF-α, cleaved caspase-3, and Bax. Bcl-2 and anti-inflammatory cytokine anti-interleukin 10 (IL-10) were increased in hGMSC-CM-treated injured cells. Moreover, hGMSC-CM treatment upregulated neurotrophins anti-brain-derived neurotrophic factor (BDNF) and NT3. Western blot data of hGMSC-CM revealed the presence of neurotrophins nerve growth factor (NGF), NT3, anti-inflammatory cytokines IL-10, and transforming growth factor beta (TGF-β), suggesting their positive role to elicit neuroprotection. Our results propose that hGMSC-CM may serve as a simple and potential autologous therapeutic tool to treat motor neuron injury.
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