ArticleMolecular microbiology2018
Novel carbohydrate binding modules in the surface anchored α-amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Association between physical activity and changes in intestinal microbiota composition: A systematic review.PloS one · 2021Pooled it
- Additional Resistant Starch from One Potato Side Dish per Day Alters the Gut Microbiota but Not Fecal Short-Chain Fatty Acid Concentrations.Nutrients · 2022Trial
- Article
- α-Amylase: Its Structure, Molecular Modification, and Application in the Food Field.Foods (Basel, Switzerland) · 2026Review
- Effect of resistant starch type 5 on gut health through modulating gut microbiota.Engineering microbiology · 2026Review
- Microbiogeographic insights as keys to understanding personalized gut microbiota responses: the role ofBioscience of microbiota, food and health · 2026Review
- Important roles ofFood science and biotechnology · 2024Review
- Birthmode and environment-dependent microbiota transmission dynamics are complemented by breastfeeding during the first year.Cell host & microbe · 2024Article
- Understanding the gut microbiota by considering human evolution: a story of fire, cereals, cooking, molecular ingenuity, and functional cooperation.Microbiology and molecular biology reviews : MMBR · 2024Review
- The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch.Nature structural & molecular biology · 2024Article
- Article
- Glycogen-Degrading Activities of Catalytic Domains of α-Amylase and α-Amylase-Pullulanase Enzymes Conserved inJournal of bacteriology · 2023Article
- Article
- Metabolic pathway prediction of core microbiome based on enterotype and orotype.Frontiers in cellular and infection microbiology · 2023Article
- The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites.Frontiers in microbiology · 2023Article
- Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Selective induction of human gut-associated acetogenic/butyrogenic microbiota based on specific microbial colonization of indigestible starch granules.The ISME journal · 2022Article
- Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome.The Journal of biological chemistry · 2022Article
- CBM20CP, a novel functional protein of starch metabolism in green algae.Plant molecular biology · 2022Article
- Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale, an important butyrate-producing organism in the gut, consumes a limited range of substrates, including starch. Host consumption of resistant starch increases the abundance of E. rectale in the intestine, likely because it successfully captures the products of resistant starch degradation by other bacteria. Here, we demonstrate that the cell wall anchored starch-degrading α-amylase, Amy13K of E. rectale harbors five CBMs that all target starch with differing specificities. Intriguingly these CBMs efficiently bind to both regular and high amylose corn starch (a type of resistant starch), but have almost no affinity for potato starch (another type of resistant starch). Removal of these CBMs from Amy13K reduces the activity level of the enzyme toward corn starches by ∼40-fold, down to the level of activity toward potato starch, suggesting that the CBMs facilitate activity on corn starch and allow its utilization in vivo. The specificity of the Amy13K CBMs provides a molecular rationale for why E. rectale is able to only use certain starch types without the aid of other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.