Evidence map›Paper›PMID 29139224›Full record

ArticleJournal of biomedical materials research. Part A2018

A polyelectrolyte multilayer platform for investigating growth factor delivery modes in human liver cultures.

Christine Lin, Raimundo Romero, Lioudmila V Sorokina, Kimberly R Ballinger, Laura W Place, Matt J Kipper, Salman R Khetani

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical materials research. Part A, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Christine LinSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.ORCID 0000-0003-1072-3854
Raimundo RomeroSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.
Lioudmila V SorokinaDepartment of Bioengineering, University of Illinois at Chicago, Chicago, Illinois.
Kimberly R BallingerDepartment of Mechanical Engineering, Colorado State University, Fort Collins, Colorado.
Laura W PlaceSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.
Matt J KipperSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.
Salman R KhetaniSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.
Colorado State University · USUniversity of Illinois Chicago · US

Funding

Engineering zonal human liver functions in vitro using microfluidicsR03EB019184 · NIBIB · UNIVERSITY OF ILLINOIS AT CHICAGO · PI KHETANI, SALMAN R · 2014 to 2015
$172k
NIBIB NIH HHS R03 EB019184
6 · The paper itself

Abstract

Polyelectrolyte multilayers (PEMs) of chitosan and heparin are useful for mimicking growth factor (GF) binding to extracellular matrix (ECM) as in vivo. Here, we developed a PEM platform for delivering bound/adsorbed GFs to monocultures of primary human hepatocytes (PHHs) and PHH/non-parenchymal cell (NPC) co-cultures, which are useful for drug development and regenerative medicine. The effects of ECM protein coating (collagen I, fibronectin, and Matrigel®) and terminal PEM layer on PHH attachment/functions were determined. Then, heparin-terminated/fibronectin-coated PEMs were used to deliver varying concentrations of an adsorbed model GF, transforming growth factor β (TGFβ), to PHH monocultures while using soluble TGFβ delivery via culture medium as the conventional control. Soluble TGFβ delivery caused a severe, monotonic, and sustained downregulation of all PHH functions measured (albumin and urea secretions, cytochrome-P450 2A6 and 3A4 enzyme activities), whereas adsorbed TGFβ delivery caused transient upregulation of 3 out of 4 functions. Finally, functionally stable co-cultures of PHHs and 3T3-J2 murine embryonic fibroblasts were created on the heparin-terminated/fibronectin-coated PEMs modified with adsorbed TGFβ to elucidate similarities and differences in functional response relative to the monocultures. In conclusion, chitosan-heparin PEMs constitute a robust platform for investigating the effects of GF delivery modes on PHH monocultures and PHH/NPC co-cultures. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 971-984, 2018.

Indexed as

Drug Delivery Systems3T3 CellsAdsorptionAnimalsCell AdhesionCells, CulturedChitosanCoculture TechniquesCulture MediaExtracellular MatrixFibroblastsFibronectinsHeparinHepatocytesHumansIntercellular Signaling Peptides and ProteinsChitosanCulture MediaFibronectinsHeparinIntercellular Signaling Peptides and ProteinsPolyelectrolytesTransforming Growth Factor betaco-culturesfibroblastshepatocytestransforming growth factor-β

Identifiers

PMID29139224
PMCPMC5826882
OpenAlexW2771011849

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.