ArticleNature communications2017
Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Review
- MntR Mediates LiaSR-RegulatedMicroorganisms · 2026Article
- Acid-dependent beta-lactam resistance inmBio · 2026Article
- From Environmental Persistence to Host Adaptation: Differential Curli Regulation inMicroorganisms · 2026Review
- Osmotic stress triggers fast and reversible PMF collapse in Escherichia coli.Biophysical journal · 2026Article
- Osmotic Stress Adaptation of Poultry-AssociatedFoods (Basel, Switzerland) · 2026Article
- LuxS/AI-2 regulates phoP/phoQ by a non-canonical mechanism to enhance acid stress survival in Salmonella Typhimurium.PLoS pathogens · 2026Article
- Hijacking competitor-derived signals: RcsB/C drivesApplied and environmental microbiology · 2026Article
- A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two-component regulatory systems.The FEBS journal · 2026Article
- Amino acid decarboxylation preservesmBio · 2026Article
- The glutamate decarboxylase system (GAD2) is activated by osmotic stress in a GadR-dependent manner in Listeria monocytogenes.FEMS microbiology letters · 2026Article
- Reprogramming the EnvZ-OmpR two-component system confers ethanol tolerance in Escherichia coli by stabilizing the outer membrane and altering ferric homeostasis.PLoS genetics · 2025Article
- Article
- Article
- Direct sensing of host ferric iron by an archetype histidine kinase mediates virulence of an enteric pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Allosteric coupling activation mechanism in histidine kinases.Scientific reports · 2025Article
- The Response Regulator OmpR Negatively Controls the Expression of Genes Implicated in Tilimycin and Tilivalline Cytotoxin Production inMicroorganisms · 2025Article
- Regulatory role of the Cpx ESR in bacterial behaviours.Virulence · 2024Review
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Response ofMicroorganisms · 2024Review
16 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Unlike eukaryotes, bacteria undergo large changes in osmolality and cytoplasmic pH. It has been described that during acid stress, bacteria internal pH promptly acidifies, followed by recovery. Here, using pH imaging in single living cells, we show that following acid stress, bacteria maintain an acidic cytoplasm and the osmotic stress transcription factor OmpR is required for acidification. The activation of this response is non-canonical, involving a regulatory mechanism requiring the OmpR cognate kinase EnvZ, but not OmpR phosphorylation. Single cell analysis further identifies an intracellular pH threshold ~6.5. Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. During osmotic stress, the internal pH is above the threshold, triggering distinct OmpR-related pathways. Preventing intracellular acidification of Salmonella renders it avirulent, suggesting that acid stress pathways represent a potential therapeutic target. These results further emphasize the advantages of single cell analysis over studies of population averages.
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