ArticleScientific reports2017
Bacillus Classification Based on Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry-Effects of Culture Conditions.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Safety Evaluation ofFood science & nutrition · 2026Article
- Performance of the LIAISON PLEX gram-positive blood culture assay for identifying bacterial pathogens and resistance genes in blood cultures.Journal of clinical microbiology · 2026Article
- Integrated Phenotypic, Proteomic (MALDI-TOF MS), and Genomic (WGS) Investigation of a Prolonged Hospital Outbreak ofAntibiotics (Basel, Switzerland) · 2026Article
- Robertmurraya beringensis Causing Late-Onset Sepsis in Neonates: A Case Series of a Hitherto Unreported Organism.Cureus · 2025Article
- Characterization of Honey Microbiome Using MALDI-TOF Mass Spectrometry and Physicochemical Study.Molecules (Basel, Switzerland) · 2025Review
- Pseudo-outbreak of Bacillaceae species associated with poor compliance with blood culture collection recommendations.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Observational
- A hotspot of diversity: novelInternational journal of systematic and evolutionary microbiology · 2024Article
- Functional and mechanistic studies of a phytogenic formulation, Shrimp Best, in growth performance and vibriosis in whiteleg shrimp.Scientific reports · 2024Article
- MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa.Scientific reports · 2024Article
- Article
- The Application of MALDI-TOF MS for a Variability Study ofVeterinary sciences · 2022Article
- Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs.Journal of applied microbiology · 2022Article
- Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis.PeerJ · 2021Article
- Pseudomonas Saliphila sp. nov., a Bacterium Isolated from Oil-Well Production Water in Qinghai Oilfield of China.Current microbiology · 2020Article
- Cold storage reveals distinct metabolic perturbations in processing and non-processing cultivars of potato (Solanum tuberosum L.).Scientific reports · 2020Article
- Article
- Identification of Bacillus species: Implication on the quality of probiotic formulations.PloS one · 2019Article
- The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland.International journal of environmental research and public health · 2018Article
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Abstract
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a reliable and rapid technique applied widely in the identification and classification of microbes. MALDI-TOF MS has been used to identify many endospore-forming Bacillus species; however, endospores affect the identification accuracy when using MALDI-TOF MS because they change the protein composition of samples. Since culture conditions directly influence endospore formation and Bacillus growth, in this study we clarified how culture conditions influence the classification of Bacillus species by using MALDI-TOF MS. We analyzed members of the Bacillus subtilis group and Bacillus cereus group using different incubation periods, temperatures and media. Incubation period was found to affect mass spectra due to endospores which were observed mixing with vegetative cells after 24 hours. Culture temperature also resulted in different mass spectra profiles depending on the temperature best suited growth and sporulation. Conversely, the four common media for Bacillus incubation, Luria-Bertani agar, nutrient agar, plate count agar and brain-heart infusion agar did not result in any significant differences in mass spectra profiles. Profiles in the range m/z 1000-3000 were found to provide additional data to the standard ribosomal peptide/protein region m/z 3000-15000 profiles to enable easier differentiation of some highly similar species and the identification of new strains under fresh culture conditions. In summary, control of culture conditions is vital for Bacillus identification and classification by MALDI-TOF MS.
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