ArticleScientific reports2017
Differences in mitochondrial function and morphology during cooling and rewarming between hibernator and non-hibernator derived kidney epithelial cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Mitochondrial membrane potential and delayed graft function following kidney transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2019Trial
- Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy.Science advances · 2026Article
- Effect of post-weaning diet on hibernation occurrence in Syrian hamsters.Experimental animals · 2026Article
- Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death.bioRxiv : the preprint server for biology · 2024Article
- Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator.Cell death & disease · 2024Article
- Article
- Cold resistance of mammalian hibernators ∼ a matter of ferroptosis?Frontiers in physiology · 2024Review
- Inhibition of Ferroptosis Enables Safe Rewarming of HEK293 Cells following Cooling in University of Wisconsin Cold Storage Solution.International journal of molecular sciences · 2023Article
- Platelets from 13-lined ground squirrels are resistant to cold storage lesions.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Mitochondrial respiration in rats during hypothermia resulting from central drug administration.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022Article
- Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.Molecular medicine (Cambridge, Mass.) · 2022Article
- Recovery from cold-induced mitochondrial fission in endothelial cells requires reconditioning temperatures of ≥ 25◦C.Frontiers in transplantation · 2022Article
- Cytoskeletal Arrest: An Anoxia Tolerance Mechanism.Metabolites · 2021Review
- Hepatic resistance to cold ferroptosis in a mammalian hibernator Syrian hamster depends on effective storage of diet-derived α-tocopherol.Communications biology · 2021Article
- SIRT3, a metabolic target linked to ataxia-telangiectasia mutated (ATM) gene deficiency in diffuse large B-cell lymphoma.Scientific reports · 2020Article
- Hibernator-Derived Cells Show Superior Protection and Survival in Hypothermia Compared to Non-Hibernator Cells.International journal of molecular sciences · 2020Article
- Research Progress of the Application of Hypothermia in the Eye.Oxidative medicine and cellular longevity · 2020Review
- Molecular strategies used by hibernators: Potential therapeutic directions for ischemia reperfusion injury and preservation of human donor organs.Transplantation reviews (Orlando, Fla.) · 2020Review
- Protection of the transplant kidney during cold perfusion with doxycycline: proteomic analysis in a rat model.Proteome science · 2020Article
- The Torpid State: Recent Advances in Metabolic Adaptations and Protective MechanismsFrontiers in physiology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hibernators show superior resistance to ischemia and hypothermia, also outside the hibernation season. Therefore, hibernation is a promising strategy to decrease cellular damage in a variety of fields, such as organ transplantation. Here, we explored the role of mitochondria herein, by comparing epithelial cell lines from a hibernator (hamster kidney cells, HaK) and a non-hibernator (human embryonic kidney cells, HEK293) during cold preservation at 4 °C and rewarming. Cell survival (Neutral Red), ATP and MDA levels, mitochondrial membrane potential (MMP), mitochondrial morphology (using fluorescent probes) and metabolism (seahorse XF) were assessed. Hypothermia induced dispersion of the tubular mitochondrial network, a loss of MMP, increased oxygen radical (MDA) and decreased ATP production in HEK293. In contrast, HaK maintained MMP and ATP production without an increase in oxygen radicals during cooling and rewarming, resulting in superior cell survival compared to HEK293. Further, normothermic HaK showed a dispersed mitochondrial network and higher respiratory and glycolysis capacity compared to HEK293. Disclosing the mechanisms that hibernators use to counteract cell death in hypothermic and ischemic circumstances may help to eventually improve organ preservation in a variety of fields, including organ transplantation.
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