Evidence map›Paper›PMID 29137147›Full record

ArticlePlants (Basel, Switzerland)2017

Development of pGEMINI, a Plant Gateway Destination Vector Allowing the Simultaneous Integration of Two cDNA via a Single LR-Clonase Reaction.

Marino Exposito-Rodriguez, Philippe P Laissue, Patricia E López-Calcagno, Philip M Mullineaux, Christine A Raines, Andrew J Simkin

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Feeding the world: impacts of elevated [COHorticulture research · 2023
    Article
  2. Article
  3. Review
  4. Fruit Development in Sweet Cherry.Plants (Basel, Switzerland) · 2022
    Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marino Exposito-RodriguezSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK. mexpos@essex.ac.uk.
Philippe P LaissueSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK. plaissue@essex.ac.uk.
Patricia E López-CalcagnoSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK. pelope@essex.ac.uk.
Philip M MullineauxSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK. mullin@essex.ac.uk.ORCID 0000-0002-1998-3540
Christine A RainesSchool of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK. rainc@essex.ac.uk.ORCID 0000-0001-7997-7823
Andrew J SimkinGenetics, Genomics and Breeding, NIAB EMR, New Road, East Malling, Kent ME19 6BJ, UK. andrew.simkin@emr.ac.uk.ORCID 0000-0001-5056-1306

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gateway technology has been used to facilitate the generation of a large number of constructs for the modification of plants for research purposes. However, many of the currently available vectors only allow the integration of a single cDNA of interest into an expression clone. The ability to over-express multiple genes in combination is essential for the study of plant development where several transcripts have a role to play in one or more metabolic processes. The tools to carry out such studies are limited, and in many cases rely on the incorporation of cDNA into expression systems via conventional cloning, which can be both time consuming and laborious. To our knowledge, this study reports on the first development of a vector allowing the simultaneous integration of two independent cDNAs via a single LR-clonase reaction. This vector "

Indexed as

fluorescencegatewaygene stackingLR clonasepGEMINI

Identifiers

PMID29137147
PMCPMC5750631

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.