ArticlePloS one2017
drLumi: An open-source package to manage data, calibrate, and conduct quality control of multiplex bead-based immunoassays data analysis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 44 citations in OpenAlex.
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- Helminth coinfections mitigate clinical, parasitological, and immune outcomes in Mozambican children with malaria.BMC medicine · 2026Article
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- Inflammation and immune activation are associated with risk of Mycobacterium tuberculosis infection in BCG-vaccinated infants.Nature communications · 2022Article
- C1q deletion exacerbates stress-induced learned helplessness behavior and induces neuroinflammation in mice.Translational psychiatry · 2022Article
- Standardized Whole Blood Assay and Bead-Based Cytokine Profiling Reveal Commonalities and Diversity of the Response to Bacteria and TLR Ligands in Cattle.Frontiers in immunology · 2022Article
- Maternal and neonatal immune response to SARS-CoV-2, IgG transplacental transfer and cytokine profile.Frontiers in immunology · 2022Article
- Stimulation of angiotensin II receptor 2 preserves cognitive function and is associated with an enhanced cerebral vascular density after stroke.Vascular pharmacology · 2021Article
- Type 1 interferon mediates chronic stress-induced neuroinflammation and behavioral deficits via complement component 3-dependent pathway.Molecular psychiatry · 2021Article
- A Tryptophan-Deficient Diet Induces Gut Microbiota Dysbiosis and Increases Systemic Inflammation in Aged Mice.International journal of molecular sciences · 2021Article
- Cutting Edge: The RNA-Binding Protein Ewing Sarcoma Is a Novel Modulator of Lymphotoxin β Receptor Signaling.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti.Scientific reports · 2020Article
- Antibody responses to the RTS,S/AS01NPJ vaccines · 2020Article
- Serum proteomic analysis of melanoma patients with immunohistochemical profiling of primary melanomas and cultured cells: Pilot study.Oncology reports · 2019Article
- Optimisation and standardisation of a multiplex immunoassay of diverseWellcome open research · 2019Article
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Authors and funding
15 authors at 7 institutions in 7 countries.
Funding
Abstract
Multiplex bead-based immunoassays are used to measure concentrations of several analytes simultaneously. These assays include control standard curves (SC) to reduce between-plate variability and normalize quantitation of analytes of biological samples. Suboptimal calibration might result in large random error and decreased number of samples with analyte concentrations within the limits of quantification. Suboptimal calibration may be a consequence of poor fitness of the functions used for the SC, the treatment of the background noise and the method used to estimate the limits of quantification. Currently assessment of fitness of curves is largely dependent on operator and that may add additional error. Moreover, there is no software to automate data managing and quality control. In this article we present a R package, drLumi, with functions for managing data, calibrating assays and performing quality control. To optimize the assay the package implements: i) three dose-response functions, ii) four approaches for treating background noise and iii) three methods for estimating limits of quantifications. Other implemented functions are focused on the quality control of the fitted standard curve: detection of outliers, estimation of the confidence or prediction interval, and estimation of summary statistics. With demonstration purpose, we apply the software to 30 cytokines, chemokines and growth factors measured in a multiplex bead-based immunoassay in a study aiming to measure correlates of risk or protection from malaria of the RTS,S malaria vaccine nested in the Phase 3 randomized controlled trial of this vaccine.
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