ArticlePloS one2017
Morphological adaptations in breast cancer cells as a function of prolonged passaging on compliant substrates.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Tumor Biomechanics-Inspired Future Medicine.Cancers · 2024Review
- Genetic variation drives cancer cell adaptation to ECM stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Extracellular matrix stiffness modulates the mechanophenotypes and focal adhesions of colon cancer cells leading to their invasions via YAP1.Mechanobiology in medicine · 2024Article
- Engineering approaches for understanding mechanical memory in cancer metastasis.APL bioengineering · 2024Review
- Small Extracellular Vesicles Promote Stiffness-mediated Metastasis.Cancer research communications · 2024Article
- Serial Passaging Affects Stromal Cell Mechanosensitivity on Hyaluronic Acid Hydrogels.Macromolecular bioscience · 2024Article
- Mechanobiology of cancer cell responsiveness to chemotherapy and immunotherapy: Mechanistic insights and biomaterial platforms.Advanced drug delivery reviews · 2023Review
- Serial passaging affects stromal cell mechanosensitivity on hyaluronic acid hydrogels.bioRxiv : the preprint server for biology · 2023Article
- Actin crosslinking by α-actinin averts viscous dissipation of myosin force transmission in stress fibers.iScience · 2023Article
- Reverse Plasticity Underlies Rapid Evolution by Clonal Selection within Populations of Fibroblasts Propagated on a Novel Soft Substrate.Molecular biology and evolution · 2021Article
- Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy.STAR protocols · 2021Article
- Adipose stem cells exhibit mechanical memory and reduce fibrotic contracture in a rat elbow injury model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Matrix stiffness-sensitive long noncoding RNA NEAT1 seeded paraspeckles in cancer cells.Molecular biology of the cell · 2020Article
- Stiffness Sensing by Cells.Physiological reviews · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Standard tissue culture practices involve propagating cells on tissue culture polystyrene (TCP) dishes, which are flat, 2-dimensional (2D) and orders of magnitude stiffer than most tissues in the body. Such simplified conditions lead to phenotypical cell changes and altered cell behaviors. Hence, much research has been focused on developing novel biomaterials and culture conditions that more closely emulate in vivo cell microenvironments. In particular, biomaterial stiffness has emerged as a key property that greatly affects cell behaviors such as adhesion, morphology, proliferation and motility among others. Here we ask whether cells that have been conditioned to TCP, would still show significant dependence on substrate stiffness if they are first pre-adapted to a more physiologically relevant environment. We used two commonly utilized breast cancer cell lines, namely MDA-MB-231 and MCF-7, and examined the effect of prolonged cell culturing on polyacrylamide substrates of varying compliance. We followed changes in cell adhesion, proliferation, shape factor, spreading area and spreading rate. After pre-adaptation, we noted diminished differences in cell behaviors when comparing between soft (1 kPa) and stiff (103 kPa) gels as well as rigid TCP control. Prolonged culturing of cells on complaint substrates further influenced responses of pre-adapted cells when transferred back to TCP. Our results have implications for the study of stiffness-dependent cell behaviors and indicate that cell pre-adaptation to the substrate needs consideration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.