ArticleJournal of the American Chemical Society2017
Hierarchical Self-Assembly of a Copolymer-Stabilized Coacervate Protocell.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed.
- Elucidating the Reciprocal Interplay between Supramolecular Polymer Cytoskeletons and Fuel-Dependent Synthetic Cells.Journal of the American Chemical Society · 2026Article
- Patchy Membrane-Directed Multiphase Complex Coacervation.Journal of the American Chemical Society · 2026Article
- Anomalous Liquid-Liquid Phase Separation Dynamics in Polymerization-Driven Complex Coacervation.ACS macro letters · 2026Article
- Oppositely Charged Single Enzyme Nanogels Form Versatile Coacervates for Efficient Enzyme Cascade Catalysis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Engineering a Transmembrane Receptor for Coacervate-Based Artificial Cells.Journal of the American Chemical Society · 2026Article
- Electrostatic bimodality of human cytosolic DNA sensor cGAS-dsDNA condensates revealed by millisecond flashing electrophoretic separation.Nature communications · 2026Article
- Bottom-Up Coacervate-Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life-Like Systems.Angewandte Chemie (International ed. in English) · 2026Review
- Reconfiguration of Multiphase Coacervate Droplets Into Self-Regulated Nested Artificial Cells.Angewandte Chemie (International ed. in English) · 2026Article
- Micropatterned DNA Hydrogels for Spatiotemporal Programming of Chemical Reaction Networks.ACS nano · 2026Article
- Dynamic Covalent Boronate Chemistry forJournal of the American Chemical Society · 2026Article
- Preservation of Complex Multiphase Architectures in Polymer-Based Artificial Cells by Photo-Crosslinking.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bottom-up Strategies for Generating Polymer Protocells That Mimic Cellular Communication.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- Complex Coacervate Emulsions as a Strategy to Stabilize Enzymes for Catalysis in Organic Solvents.ACS macro letters · 2026Article
- Design and applications of synthetic biomolecular condensates.Nature nanotechnology · 2026Review
- Automated navigation of condensate phase behavior with active machine learning.Nature communications · 2025Article
- Photoactivatable Synthetic Exosomes for RNA-Based Communication Between Artificial Cells and Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- A Photonastic Prototissue Capable of Photo-Mechano-Chemical Transduction.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Engineering Motile Coacervate Droplets via Nanomotor Stabilization.Journal of the American Chemical Society · 2025Article
- Merging Natural Biopolymers with Supramolecular Chemistry: Emulating the Native Extracellular Matrix's Complexity.ACS nano · 2025Review
51 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complex coacervate microdroplets are finding increased utility in synthetic cell applications due to their cytomimetic properties. However, their intrinsic membrane-free nature results in instability that limits their application in protocell research. Herein, we present the development of a new protocell model through the spontaneous interfacial self-assembly of copolymer molecules on biopolymer coacervate microdroplets. This hierarchical protocell model not only incorporates the favorable properties of coacervates (such as spontaneous assembly and macromolecular condensation) but also assimilates the essential features of a semipermeable copolymeric membrane (such as discretization and stabilization). This was accomplished by engineering an asymmetric, biodegradable triblock copolymer molecule comprising hydrophilic, hydrophobic, and polyanionic components capable of direct coacervate membranization via electrostatic surface anchoring and chain self-association. The resulting hierarchical protocell demonstrated striking integrity as a result of membrane formation, successfully stabilizing enzymatic cargo against coalescence and fusion in discrete protocellular populations. The semipermeable nature of the copolymeric membrane enabled the incorporation of a simple enzymatic cascade, demonstrating chemical communication between discrete populations of neighboring protocells. In this way, we pave the way for the development of new synthetic cell constructs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.