Evidence map›Paper›PMID 29134471›Full record

ArticleCardiovascular engineering and technology2018

The Impact of Heat Treatment on Porcine Heart Valve Leaflets.

R Glenn Hepfer, Peng Chen, Kelvin G M Brockbank, Alyce L Jones, Amanda K Burnette, Zhen Chen, Elizabeth D Greene, Lia H Campbell, Hai Yao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular engineering and technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

R Glenn HepferDepartment of Bioengineering, Clemson-MUSC Bioengineering Program, Clemson University, 173 Ashley Avenue, MSC 508, Charleston, SC, 29425, USA.
Peng ChenDepartment of Bioengineering, Clemson-MUSC Bioengineering Program, Clemson University, 173 Ashley Avenue, MSC 508, Charleston, SC, 29425, USA.
Kelvin G M BrockbankDepartment of Bioengineering, Clemson-MUSC Bioengineering Program, Clemson University, 173 Ashley Avenue, MSC 508, Charleston, SC, 29425, USA.
Alyce L JonesLifeNet Health, Virginia Beach, VA, USA.
Amanda K BurnetteLifeNet Health, Virginia Beach, VA, USA.
Zhen ChenTissue Testing Technologies LLC, North Charleston, SC, USA.
Elizabeth D GreeneTissue Testing Technologies LLC, North Charleston, SC, USA.
Lia H CampbellTissue Testing Technologies LLC, North Charleston, SC, USA.
Hai YaoDepartment of Bioengineering, Clemson-MUSC Bioengineering Program, Clemson University, 173 Ashley Avenue, MSC 508, Charleston, SC, 29425, USA. haiyao@clemson.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this study was to determine the impact of elevated temperature exposure in tissue banking on soft tissues. A secondary objective was to determine the relative ability of various assays to detect changes in soft tissues due to temperature deviations. Porcine pulmonary heart valve leaflets exposed to 37 °C were compared with those incubated at 52 and 67 °C for 10, 30 and 100 min. The analytical methods consisted of (1) viability assessment using the resazurin assay, (2) collagen content using the Sircol assay, and (3) permeability assessment using an electrical conductivity assay. Additionally, histology and two photon microscopy were used to reveal mechanisms of cell and tissue damage. Viability, collagen content, and permeability all decreased following heat treatment. In terms of statistical significance with respect to treatment temperature, cell viability was most affected (p < 0.0001), followed by permeability (p < 0.0001), and then collagen content (p = 0.13). After heat treatment, histology indicated increased apoptosis and two photon microscopy revealed a decrease in collagen fiber organization and an increase in elastin density. These results suggest that measures of cell viability would be best for assessing tissues where the cells are alive and that permeability may be best where cell viability is not intentionally maintained.

Indexed as

BioprosthesisHeart Valve ProsthesisHot TemperatureAnimalsApoptosisCell SurvivalElastinExtracellular MatrixFibrillar CollagensHeart Valve Prosthesis ImplantationIn Situ Nick-End LabelingMicroscopy, Fluorescence, MultiphotonPermeabilityPulmonary ValveSus scrofaTime FactorsElastinFibrillar CollagensCell viabilityCollagen contentHeart valveHeat treatmentMatrix permeabilityTissue banking

Identifiers

PMID29134471
PMCPMC5797199

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.