ArticleMalaria journal2017
Yeast interfering RNA larvicides targeting neural genes induce high rates of Anopheles larval mortality.
Article in Malaria journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
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- Maximizing the Potential of Attractive Targeted Sugar Baits (ATSBs) for Integrated Vector Management.Insects · 2023Review
- Mosquito gene targeted RNAi studies for vector control.Functional & integrative genomics · 2023Review
- MosChito rafts as effective and eco-friendly tool for the delivery of a Bacillus thuringiensis-based insecticide to Aedes albopictus larvae.Scientific reports · 2023Article
- Effect of marker-free transgenic Chlamydomonas on the control of Aedes mosquito population and on plankton.Parasites & vectors · 2023Article
- Field trials reveal the complexities of deploying and evaluating the impacts of yeast-baited ovitraps on Aedes mosquito densities in Trinidad, West Indies.Scientific reports · 2022Article
- A Conserved Female-Specific Requirement for thePathogens (Basel, Switzerland) · 2022Article
- RNAi-based pest control: Production, application and the fate of dsRNA.Frontiers in bioengineering and biotechnology · 2022Review
- Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides.Frontiers in bioengineering and biotechnology · 2022Review
- A Yeast RNA-Interference Pesticide Targeting theInsects · 2021Article
- MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens.Parasites & vectors · 2021Article
- A Broad-Based Mosquito Yeast Interfering RNA Pesticide TargetingPathogens (Basel, Switzerland) · 2021Article
- Development of an RNAi-based microalgal larvicide for the control of Aedes aegypti.Parasites & vectors · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough larviciding can reduce the number of outdoor biting malaria vector mosquitoes, which may help to prevent residual malaria transmission, the current larvicide repertoire is faced with great challenges to sustainability. The identification of new effective, economical, and biorational larvicides could facilitate maintenance and expansion of the practice of larviciding in integrated malaria vector mosquito control programmes. Interfering RNA molecules represent a novel class of larvicides with untapped potential for sustainable mosquito control. This investigation tested the hypothesis that short interfering RNA molecules can be used as mosquito larvicides.
resultsA small interfering RNA (siRNA) screen for larval lethal genes identified siRNAs corresponding to the Anopheles gambiae suppressor of actin (Sac1), leukocyte receptor complex member (lrc), and offtrack (otk) genes. Saccharomyces cerevisiae (baker's yeast) was engineered to produce short hairpin RNAs (shRNAs) for silencing of these genes. Feeding larvae with the engineered yeasts resulted in silenced target gene expression, a severe loss of neural synapses in the larval brain, and high levels of larval mortality. The larvicidal activities of yeast interfering RNA larvicides were retained following heat inactivation and drying of the yeast into user-friendly tablet formulations that induced up to 100% larval mortality in laboratory trials.
conclusionsReady-to-use dried inactivated yeast interfering RNA larvicide tablets may someday be an effective and inexpensive addition to malaria mosquito control programmes and a valuable, biorational tool for addressing residual malaria transmission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.