ArticleBMC plant biology2017
Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Transgenic expression of mAChR-C dsRNA in maize confers efficient locust control.Plant communications · 2025Article
- Risk Assessment of RNAi-Based Potential Pesticide dsInsects · 2025Article
- Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum.Journal of nanobiotechnology · 2025Article
- Nanocarrier-Based Eco-Friendly RNA Pesticides for Sustainable Management of Plant Pathogens and Pests.Nanomaterials (Basel, Switzerland) · 2024Review
- Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall Armyworms.Bio-protocol · 2023Article
- Plastid Transformation of Micro-Tom Tomato with a Hemipteran Double-Stranded RNA Results in RNA Interference in Multiple Insect Species.International journal of molecular sciences · 2022Article
- Five Visual and Olfactory Target Genes for RNAi inFrontiers in genetics · 2022Article
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA.RNA biology · 2021Article
- Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.Plant biotechnology journal · 2021Article
- Transgenic Expression of dsRNA Targeting thePlants (Basel, Switzerland) · 2021Article
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
- RNAi technology for management of banana bunchy top disease.Food and energy security · 2020Review
- Negligible transcriptome and metabolome alterations in RNAi insecticidal maize against Monolepta hieroglyphica.Plant cell reports · 2020Article
- Mechanisms, Applications, and Challenges of Insect RNA Interference.Annual review of entomology · 2020Review
- Expression and functional analysis of P450 gene induced tolerance/resistance to lambda-cyhalothrin in quercetin fed larvae of beet armywormSaudi journal of biological sciences · 2020Article
- Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance.eLife · 2019Article
- Providence virus: An animal virus that replicates in plants or a plant virus that infects and replicates in animal cells?PloS one · 2019Article
- RNA Interference in the Tobacco Hornworm,Frontiers in plant science · 2019Article
- Global challenges faced by engineered3 Biotech · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant-mediated RNAi (PMRi) silencing of insect genes has enormous potential for crop protection, but whether it works robustly under field conditions, particularly with lepidopteran pests, remains controversial. Wild tobacco Nicotiana attenuata and cultivated tobacco (N. tabacum) (Solanaceae) is attacked by two closely related specialist herbivores Manduca sexta and M. quinquemaculata (Lepidoptera, Sphingidae). When M. sexta larvae attack transgenic N. attenuata plants expressing double-stranded RNA(dsRNA) targeting M. sexta's midgut-expressed genes, the nicotine-ingestion induced cytochrome P450 monooxygenase (invert repeat (ir)CYP6B46-plants) and the lyciumoside-IV-ingestion induced β-glucosidase1 (irBG1-plants), these larval genes which are important for the larvae's response to ingested host toxins, are strongly silenced.
resultsHere we show that the PMRi procedure also silences the homologous genes in native M. quinquemaculata larvae feeding on irCYP6B46 and irBG1-transgenic N. attenuata plants in nature. The PMRi lines shared 98 and 96% sequence similarity with M. quinquemaculata homologous coding sequences, and CYP6B46 and BG1 transcripts were reduced by ca. 90 and 80%, without reducing the transcripts of the larvae's most similar, potential off-target genes.
conclusionsWe conclude that the PMRi procedure can robustly and specifically silence genes in native congeneric insects that share sufficient sequence similarity and with the careful selection of targets, might protect crops from attack by congeneric-groups of insect pests.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.