ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2018
Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.
Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Expression of MHC I Isoforms in Bovine Placentomes: Impact of Cloning.Veterinary sciences · 2025Article
- IFN-γ in ovarian tumor microenvironment upregulates HLA-E expression and predicts a poor prognosis.Journal of ovarian research · 2023Article
- Blood transcriptome reveals immune and metabolic-related genes involved in growth of pasteurized colostrum-fed calves.Frontiers in genetics · 2023Article
- Increased expression of pro-inflammatory cytokines at the fetal-maternal interface in bovine pregnancies produced by cloning.American journal of reproductive immunology (New York, N.Y. : 1989) · 2022Article
- Development and characterization of type I interferon receptor knockout sheep: A model for viral immunology and reproductive signaling.Frontiers in genetics · 2022Article
- DNA methylation studies in cattle.Journal of applied genetics · 2021Review
- Integrated Analysis of FAM57A Expression and Its Potential Roles in Hepatocellular Carcinoma.Frontiers in oncology · 2021Article
- Article
- Promotion on NLRC5 upregulating MHC-I expression by IFN-γ in MHC-I-deficient breast cancer cells.Immunologic research · 2019Article
- Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data.Scientific reports · 2019Article
- Reduced bonobo MHC class I diversity predicts a reduced viral peptide binding ability compared to chimpanzees.BMC evolutionary biology · 2019Article
- Identification of DNA methylation-driven genes in esophageal squamous cell carcinoma: a study based on The Cancer Genome Atlas.Cancer cell international · 2019Article
- Identification of candidate aberrantly methylated and differentially expressed genes in thyroid cancer.Journal of cellular biochemistry · 2018Article
- Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.American journal of reproductive immunology (New York, N.Y. : 1989) · 2018Article
- DNA methylation biomarkers for hepatocellular carcinoma.Cancer cell international · 2018Article
- Immunological Homeostasis at the Ovine Placenta May Reflect the Degree of Maternal Fetal Interaction.Frontiers in immunology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
problemThe regulatory mechanisms governing differential expression of classical major histocompatibility complex (MHC) class I (MHC-Ia) and non-classical MHC class I (MHC-Ib) genes are poorly understood. METHOD OF STUDY: Quantitative reverse transcription- polymerase chain reaction (PCR) was used to compare the abundance of MHC-I transcripts and related transcription factors in peripheral blood mononuclear cells (PBMC) and placental trophoblast cells (PTC). Methylation of MHC-I CpG islands was detected by bisulfite treatment and next-generation sequencing. Demethylation of PBMC and PTC with 5'-aza-deoxycytidine was used to assess the role of methylation in gene regulation.
resultsMHC-I expression was higher in PBMC than PTC and was correlated with expression of IRF1, class II MHC transactivator (CIITA), and STAT1. The MHC-Ia genes and BoLA-NC1 were devoid of CpG methylation in PBMC and PTC. In contrast, CpG sites in the gene body of BoLA-NC2, -NC3, and -NC4 were highly methylated in PBMC but largely unmethylated in normal PTC and moderately methylated in somatic cell nuclear transfer PTC. In PBMC, demethylation resulted in upregulation of MHC-Ib by 2.8- to 6-fold, whereas MHC-Ia transcripts were elevated less than 2-fold.
conclusionDNA methylation regulates bovine MHC-Ib expression and is likely responsible for the different relative levels of MHC-Ib to MHC-Ia transcripts in PBMC and PTC.
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