ArticleBiotechnology and bioengineering2018
Genome-wide identification of tolerance mechanisms toward p-coumaric acid in Pseudomonas putida.
Article in Biotechnology and bioengineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 91 citations in OpenAlex.
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- Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5.Environmental microbiology reports · 2025Article
- High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance.Environmental microbiology · 2025Article
- UpgradingACS omega · 2025Article
- Development of genetic manipulation tools forFrontiers in microbiology · 2025Article
- Maintenance of bacterial outer membrane lipid asymmetry: insight into MlaA.BMC microbiology · 2024Review
- Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules inmSystems · 2024Article
- Engineering a Pseudomonas taiwanensis 4-coumarate platform for production of para-hydroxy aromatics with high yield and specificity.Metabolic engineering · 2023Article
- Role of the CrcB transporter of Pseudomonas putida in the multi-level stress response elicited by mineral fluoride.Environmental microbiology · 2022Article
- Bacterial Community Assembly, Succession, and Metabolic Function during Outdoor Cultivation ofmSphere · 2022Article
- Global transcriptomic response of Escherichia coli to p-coumaric acid.Microbial cell factories · 2022Article
- Experimental and Analytical Approaches for Improving the Resolution of Randomly Barcoded Transposon Insertion Sequencing (RB-TnSeq) Studies.ACS synthetic biology · 2022Article
- Characterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.Applied and environmental microbiology · 2022Article
- Unraveling the mechanism of furfural tolerance in engineeredFrontiers in microbiology · 2022Article
- When metabolic prowess is too much of a good thing: how carbon catabolite repression and metabolic versatility impede production of esterified α,ω-diols in Pseudomonas putida KT2440.Biotechnology for biofuels · 2021Article
- The Pseudomonas aeruginosa substrate-binding protein Ttg2D functions as a general glycerophospholipid transporter across the periplasm.Communications biology · 2021Article
- One major facilitator superfamily transporter is responsible for propionic acid tolerance in Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- Adaptive laboratory evolution ofMetabolic engineering communications · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found toward p-coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome-wide approach, Tn-seq. Libraries containing a large number of miniTn5-Km transposon insertion mutants were grown in the presence and absence of p-coumaric acid, and the enrichment or depletion of mutants was quantified by high-throughput sequencing. Several genes, including the ABC transporter Ttg2ABC and the cytochrome c maturation system (ccm), were identified to play an important role in the tolerance toward p-coumaric acid of this bacterium. Most of the identified genes were involved in membrane stability, suggesting that tolerance toward p-coumaric acid is related to transport and membrane integrity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.