Evidence map›Paper›PMID 29131209›Full record

ArticleThe Analyst2017

Bulk double emulsification for flow cytometric analysis of microfluidic droplets.

David J Sukovich, Samuel C Kim, Noorsher Ahmed, Adam R Abate

Abstract read
In one paragraph

Article in The Analyst, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey.Angewandte Chemie (International ed. in English) · 2021
    Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David J SukovichDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158, USA. adam@abatelab.org.
Samuel C Kim
Noorsher Ahmed
Adam R Abate

Funding

Gigapixel digital PCR in Giant Unilamellar VesiclesR01EB019453 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., CHIU, CHARLES YEN · 2014 to 2022
$3.9M
Microfluidic immunoprofiling for biomarker discovery in rheumatoid arthritisDP2AR068129 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2014 to 2014
$2.4M
Accurate and unbiased single cell sequencing with digital droplet MDAR01HG008978 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2016 to 2018
$1.9M
SINGLE-CELL TRANSCRIPTOMIC ANALYSIS OF HIV-INFECTED CD4 T CELLS IN BLOOD AND LYMPHOID TISSUE USING PCR-ACTIVATED CELL SORTINGU01AI129206 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., BORITZ, ELI A · 2017 to 2020
$1.6M
Evaluating HIV expression and latency in blood and tissues at the single cell levelR21AI116218 · NIAID · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI WONG, JOSEPH K · 2014 to 2015
$442k
NHGRI NIH HHS R01 HG008978NIAID NIH HHS R21 AI116218NIAID NIH HHS U01 AI129206NIAMS NIH HHS DP2 AR068129NIBIB NIH HHS R01 EB019453
6 · The paper itself

Abstract

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

Identifiers

PMID29131209
PMCPMC5997486

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.