ArticleNature methods2017
The 3D OrbiSIMS-label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 186 papers.
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Who cites it
186 citing papers in PubMed.
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- TOF-SIMS Imaging of Intact Lipids in Biological Tissue with Enhanced Ion Intensity by Ionic Liquid.ACS omega · 2026Article
- Insights From Spatiality: Accelerating Pharmaceutical Research Using Mass Spectrometry Imaging.Medicinal research reviews · 2026Review
- Burn-in-Loss-Free Organic Solar Cells With Over 2000 h of Thermal Stability Through Nitrone-Based Interface Passivation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Spatial multi-omics analyses of the tumor immune microenvironment: advances and novel therapeutic avenues.Molecular cancer · 2026Review
- Dissecting human organ development using spatial technologies.Developmental cell · 2026Review
- Advances in spatial omics for the analysis of prostate cancer.Analytical and bioanalytical chemistry · 2026Review
- Targeting the Lipid Metabolism Proteins FASN and GPAM in Alveolar Type II Cells Decreases Lung Metastasis.Cancer discovery · 2026Article
- Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging.Nature methods · 2026Article
- Cryo-HIM-SIMS on the npSCOPE: Correlative Topographic, Transmitted and SIMS Imaging at Cryogenic Temperatures.Analytical chemistry · 2026Article
- Contemporary Techniques and Prospects in Pharmaceutical Tablet Surface Analysis.ACS measurement science au · 2026Article
- Single-Cell and Spatial Omics: Methods and Applications.MedComm · 2026Review
- In Situ Lipid Interactions of an Anticancer Metal Complex.Inorganic chemistry · 2026Article
- High-Resolution Microscope-Mode Secondary Ion Mass Spectrometry Imaging.Analytical chemistry · 2026Article
- Single-cell thiol profiling enabled by live-cell labeling reveals metabolic heterogeneity in ferroptosis.Nature communications · 2026Article
- In vivo multimodal neurochemical interfaces for real-time decoding of brain circuit.Nature reviews. Neuroscience · 2026Review
- Molecular Biointerface Characterization for an Implanted Medical Device Using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS).ACS applied materials & interfaces · 2026Article
- Glycosaminoglycans and Their Role in Capillary Walls.Advances in experimental medicine and biology · 2026Review
126 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; under 200 nm for inorganic species and under 2 μm for biomolecules) with the high mass-resolving power of an Orbitrap (>240,000 at m/z 200). This allows exogenous and endogenous metabolites to be visualized in 3D with subcellular resolution. We imaged the distribution of neurotransmitters-gamma-aminobutyric acid, dopamine and serotonin-with high spectroscopic confidence in the mouse hippocampus. We also putatively annotated and mapped the subcellular localization of 29 sulfoglycosphingolipids and 45 glycerophospholipids, and we confirmed lipid identities with tandem mass spectrometry. We demonstrated single-cell metabolomic profiling using rat alveolar macrophage cells incubated with different concentrations of the drug amiodarone, and we observed that the upregulation of phospholipid species and cholesterol is correlated with the accumulation of amiodarone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.