ArticleNature medicine2017
Enhancing the precision of genetic lineage tracing using dual recombinases.
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Article in Nature medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 175 papers, 1 of them a synthesis that pooled it.
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175citing papers in PubMed, 1 pooled it
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Who cites it
175 citing papers in PubMed, 1 synthesis or guideline pooled it, 258 citations in OpenAlex.
- Crosstalk between fibroblasts and immunocytes in fibrosis: From molecular mechanisms to clinical trials.Clinical and translational medicine · 2024Pooled it
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- Dual Lineage Tracing Identifies Cellular Mechanisms Underlying Radiation-Associated Changes in Atherosclerotic Lesion Composition-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.The EMBO journal · 2026Article
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- Translational insights and clinical challenges of targeting cancer stem cells.Signal transduction and targeted therapy · 2026Review
- Dual recombinase-mediated genetic labeling and selective ablation of fibroblasts.Science China. Life sciences · 2026Article
- Lineage tracing from cellular heritage to disease destiny.Nature genetics · 2026Review
- Cancer stem cell plasticity in shaping drug resistance landscapes in prostate cancer.Journal of advanced research · 2026Review
- Dual recombinases-mediated genetic tracing reveals no postnatal neo-oogenesis in mice.iScience · 2026Article
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- Contribution of Gli1Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Dual recombinase-mediated endothelial cell-specific lineage tracing and ablation.Cell regeneration (London, England) · 2026Article
- Mapping the Tissue-of-Origins of Mesenchymal Stromal Cells in Injury Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Endothelial-to-mesenchymal transition in atherosclerosis: mechanisms, therapeutic targets, and future perspectives.Frontiers in cell and developmental biology · 2026Review
- Lineage tracing reveals KSPFrontiers in cell and developmental biology · 2026Article
- A guide to cell-type-specific Cre mouse models in atherosclerosis: from background strains to future directions.Frontiers in cardiovascular medicine · 2026Review
- Tracing the origin of myofibroblasts in kidney fibrosis.Nature communications · 2025Article
- Pyruvate kinase M2 -mediated histone lactylation alters three-dimensional genomic architecture in polycystic ovary syndrome.Signal transduction and targeted therapy · 2025Article
115 more citing papers are in PubMed but not listed here.
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Authors and funding
27 authors at 11 institutions in 3 countries.
Lingjuan HeState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0003-2747-5148
Yan LiState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-5838-3185
Yi LiState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0001-6166-8109
Wenjuan PuState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0001-5155-5178
Xiuzhen HuangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-4087-8964
Xueying TianState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0003-1113-0057
Yue WangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-8773-8627
Hui ZhangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-7038-5407
Qiaozhen LiuState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-8303-653X
Libo ZhangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.
Huan ZhaoState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.
Juan TangState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.
Hongbin JiState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.
Dongqing CaiKey Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China.
Zhibo HanState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China.
Zhongchao HanState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China.
Yu NieState Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.ORCID http://orcid.org/0000-0002-8744-0046
Shengshou HuState Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Qing-Dong WangBioscience Heart Failure, Cardiovascular and Metabolic Diseases, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden.ORCID http://orcid.org/0000-0001-9570-4411
Ruilin SunShanghai Model Organisms Center, Inc., Shanghai, China.
Jian FeiShanghai Model Organisms Center, Inc., Shanghai, China.
Fengchao WangNational Institute of Biological Sciences, Beijing, China.
Ting ChenNational Institute of Biological Sciences, Beijing, China.
Yan YanZhongshan Hospital, Fudan University, Shanghai, China.
Hefeng HuangInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
William T PuDepartment of Cardiology, Boston Children's Hospital, Boston, Massachusetts, USA and Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
Bin ZhouState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0001-5278-5522
Shanghai Institutes for Biological Sciences · CNBoston Children's Hospital · USChinese Academy of Medical Sciences & Peking Union Medical College · CNInstitute of Hematology & Blood Diseases Hospital · CNJinan University · CNNational Institute of Biological Sciences, Beijing · CNShanghai Model Organisms (China) · CNAstraZeneca (Sweden) · SEShanghai Jiao Tong University · CNShanghaiTech University · CNSun Yat-sen University · CN
Funding
Regulation of Cardiac Development in Health and DiseaseUM1HL098166 · NHLBI · HARVARD MEDICAL SCHOOL · PI PENNACCHIO, LEN ALEXANDER, PU, WILLIAM TSWENCHING · 2015 to 2019
$4.4MEpicardial progenitors in the developing and postnatal heartR01HL094683 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI PU, WILLIAM TSWENCHING · 2009 to 2016
$3.7MUnderstanding mitochondrial regulation of cardiac development and function through studies of Barth SyndromeR01HL128694 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI PU, WILLIAM TSWENCHING · 2015 to 2018
$2.0MNHLBI NIH HHS R01 HL094683NHLBI NIH HHS R01 HL128694NHLBI NIH HHS UM1 HL098166
Abstract
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing. The Dre-rox system permits rigorous control of Cre-loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression. Using this new system we investigated two topics of recent debates-the contribution of c-Kit
Indexed as
AnimalsCell LineageCell PlasticityCell TrackingEmbryo, MammalianEscherichia coli ProteinsFemaleGene Expression Regulation, DevelopmentalGenes, ReporterIntegrasesMaleMiceMice, Inbred C57BLMice, Inbred ICRMice, TransgenicMolecular ImagingCre recombinaseDre recombinase, E coliEscherichia coli ProteinsIntegrasesRecombinases
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.