Evidence map›Paper›PMID 29131007›Full record

ArticleCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology2017

Proteinase-Activated Receptor-2 Sensitivity of Amplified TRPA1 Activity in Skeletal Muscle Afferent Nerves and Exercise Pressor Reflex in Rats with Femoral Artery Occlusion.

Jihong Xing, Jianhua Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
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  3. Neuroimmune recognition of allergens.Current opinion in immunology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Jihong XingDepartment of Emergency Medicine, The First Hospital of Jilin University, Changchun, China.
Jianhua LiPennsylvania State Heart & Vascular Institute, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.
First Hospital of Jilin University · CNPennsylvania State University · US

Funding

The Muscle Reflex: Modes of Engagement and Circulatory ConsequencesP01HL096570 · NHLBI · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI LEUENBERGER, URS A · 2010 to 2014
$9.9M
Project 3: Role Played by ASIC, P2X and EP4 Receptors in the Exercise Pressor Reflex in Health and Simulated PADP01HL134609 · NHLBI · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI SINOWAY, LAWRENCE I · 2017 to 2021
$9.4M
Sympathetic Nervous System and Heart Failure-Role of Primary Afferent NeuronsR01HL090720 · NHLBI · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI LI, JIANHUA · 2009 to 2010
$776k
NHLBI NIH HHS P01 HL096570NHLBI NIH HHS P01 HL134609NHLBI NIH HHS R01 HL090720
6 · The paper itself

Abstract

BACKGROUND/

aimsLimb ischemia occurs in peripheral artery disease (PAD). Sympathetic nerve activity (SNA) that regulates blood flow directed to the ischemic limb is exaggerated during exercise in this disease, and transient receptor potential channel A1 (TRPA1) in thin-fiber muscle afferents contributes to the amplified sympathetic response. The purpose of the present study was to determine the role of proteinase-activated receptor-2 (PAR2) in regulating abnormal TRPA1 function and the TRPA1-mediated sympathetic component of the exercise pressor reflex.

methodsA rat model of femoral artery ligation was employed to study PAD. Dorsal root ganglion (DRG) tissues were obtained to examine the protein levels of PAR2 using western blot analysis. Current responses induced by activation of TRPA1 in skeletal muscle DRG neurons were characterized using whole-cell patch clamp methods. The blood pressure response to static exercise (i.e., muscle contraction) and stimulation of TRPA1 was also examined after a blockade of PAR2.

resultsThe expression of PAR2 was amplified in DRG neurons of the occluded limb, and PAR2 activation with SL-NH2 (a PAR2 agonist) increased the amplitude of TRPA1 currents to a greater degree in DRG neurons of the occluded limb. Moreover, FSLLRY-NH2 (a PAR antagonist) injected into the arterial blood supply of the hindlimb muscles significantly attenuated the pressor response to muscle contraction and TRPA1 stimulation in rats with occluded limbs.

conclusionsThe PAR2 signal in muscle sensory nerves contributes to the amplified exercise pressor reflex via TRPA1 mechanisms in rats with femoral artery ligation. These findings provide a pathophysiological basis for autonomic responses during exercise activity in PAD, which may potentially aid in the development of therapeutic approaches for improvement of blood flow in this disease.

Indexed as

AnimalsArterial Occlusive DiseasesBlood PressureDisease Models, AnimalFemoral ArteryGanglia, SpinalHindlimbIn Vitro TechniquesMaleMuscle ContractionMuscle, SkeletalNeurons, AfferentOligopeptidesPatch-Clamp TechniquesRatsRats, Sprague-DawleyH-Phe-Ser-Leu-Leu-Arg-Tyr-NH2OligopeptidesReceptor, PAR-2seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamideTRPA1 Cation ChannelTrpa1 protein, ratHindlimb ischemiaMuscle afferent nervePAR2Peripheral arterial diseaseTRPA1

Identifiers

PMID29131007
PMCPMC5767530
OpenAlexW2767899065

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.