Evidence map›Paper›PMID 29130617›Full record

ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2018

Dendritic cell phenotype in severe asthma reflects clinical responsiveness to glucocorticoids.

E S Chambers, A M Nanzer, P E Pfeffer, D F Richards, A R Martineau, C J Griffiths, C J Corrigan, C M Hawrylowicz

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Effect of DMARDs on the immunogenicity of vaccines.Nature reviews. Rheumatology · 2023
    Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Dendritic cell phenotype in severe asthma reflects clinical responsiveness to glucocorticoids.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

E S ChambersMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.ORCID 0000-0003-0990-8835
A M NanzerMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.
P E PfefferMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.
D F RichardsMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.
A R MartineauAsthma UK Centre for Applied Research, Centre for Primary Care and Public Health, Blizard Institute, Queen Mary, University of London, London, UK.
C J GriffithsAsthma UK Centre for Applied Research, Centre for Primary Care and Public Health, Blizard Institute, Queen Mary, University of London, London, UK.
C J CorriganMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.
C M HawrylowiczMRC and Asthma-UK Centre for Allergic Mechanisms in Asthma, King's College London, London, UK.ORCID 0000-0002-2337-7463
King's College London · GBQueen Mary University of London · GB

Funding

Medical Research Council G1000758Wellcome Trust
6 · The paper itself

Abstract

backgroundSubsets of patients with severe asthma remain symptomatic despite prolonged, high-dose glucocorticoid therapy. We hypothesized that the clinical glucocorticoid sensitivity of these asthmatics is reflected in differences in peripheral blood dendritic cell subsets.

objectiveTo compare peripheral blood leucocyte populations using flow cytometry at baseline and after 2 weeks of systemic glucocorticoid (steroid) treatment to identify immunological differences between steroid-sensitive (SS) and steroid-resistant (SR) asthmatics.

methodsAdult severe asthmatics (SS n = 12; SR n = 23) were assessed for their response to 2 weeks of therapy with oral prednisolone. Peripheral blood was obtained before and after therapy and stained for lymphocyte (CD3, CD19, CD4, CD8 and Foxp3) and dendritic cell markers (Lineage negative [CD3, CD14, CD16, CD19, CD20, CD56], HLA-DR+, CD304, CD11c, ILT3 and CD86).

resultsA higher median frequency of myeloid DCs (mDCs) but not plasmacytoid DCs (pDCs) was observed in the blood of SR as compared to SS asthmatics (P = .03). Glucocorticoid therapy significantly increased median B cell, but not T cell numbers in both cohorts, with a trend for increased numbers of Foxp3+ Tregs in SS (P = .07), but not SR subjects. Oral prednisolone therapy significantly reduced the median numbers and frequencies of total DCs and pDCs in both SS and SR asthmatics. Interestingly, the expression of HLA-DR and ILT3 was also reduced on pDCs in all patients. In contrast, therapy increased the median frequency of mDCs in SS, but reduced it in SR asthmatics.

conclusionsMyeloid DC frequency is elevated in SR compared with SS asthmatics, and mDC shows a differential response to oral prednisolone therapy.

Indexed as

Administration, OralAdultAntigens, CDAsthmaDendritic CellsFemaleFlow CytometryGlucocorticoidsHumansMalePrednisoloneT-LymphocytesAntigens, CDGlucocorticoidsPrednisoloneasthmadendritic cellglucocorticoidsteroid resistantsteroid sensitiveT cells

Identifiers

PMID29130617
PMCPMC5767735
OpenAlexW2767797502

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.