ArticleMethods in molecular biology (Clifton, N.J.)2018
Single mRNA Molecule Detection in Drosophila.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Ribozyme-Mediated Knockdown of lncRNA Gene Expression inBio-protocol · 2025Article
- Visualization of the Cytoskeleton in Fixed Drosophila Embryos.Current protocols · 2025Article
- The homie insulator has sub-elements with different insulating and long-range pairing properties.Genetics · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
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- Chromosome structure ineLife · 2024Article
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- Germline/soma distinction ineLife · 2023Article
- Review
- Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Frazzled/Dcc acts independently of Netrin to promote germline survival during Drosophila oogenesis.Development (Cambridge, England) · 2021Article
- Article
- CLAMP and Zelda function together to promoteeLife · 2021Article
- Correct dosage of X chromosome transcription is controlled by a nuclear pore component.Cell reports · 2021Article
- Antagonism betweeneLife · 2021Article
- A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development.Communications biology · 2020Article
- The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts.eLife · 2020Article
- Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation.Methods in enzymology · 2020Article
- Article
- Review
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Authors and funding
2 authors.
Funding
Abstract
Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.
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Registered trials
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