Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases.

Daniel Shin, Rajesh Nagarajan

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

In vitro synthesis and biochemical characterization of acyl-homoserine lactone synthase and its deletion mutant.PloS one · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Daniel ShinDepartment of Chemistry and Biochemistry, Boise State University, 1910 University Drive, Boise, ID, 83725, USA.

Rajesh NagarajanDepartment of Chemistry and Biochemistry, Boise State University, 1910 University Drive, Boise, ID, 83725, USA. rajnagarajan@boisestate.edu.

Funding

Acyl-homoserine lactone signal fidelity enforcing mechanism in bacterial communicationR15GM117323 · NIGMS · BOISE STATE UNIVERSITY · PI NAGARAJAN, RAJESH · 2016 to 2016

$396k

NIGMS NIH HHS R15 GM117323

6 · The paper itself

Abstract

Bacteria use chemical molecules called autoinducers as votes to poll their numerical strength in a colony. This polling mechanism, commonly referred to as quorum sensing, enables bacteria to build a social network and provide a collective response for fighting off common threats. In Gram-negative bacteria, AHL synthases synthesize acyl-homoserine lactone (AHL) autoinducers to turn on the expression of several virulent genes including biofilm formation, protease secretion, and toxin production. Therefore, inhibiting AHL signal synthase would limit quorum sensing and virulence. In this chapter, we describe four enzymatic methods that could be adopted to investigate a broad array of AHL synthases. The enzymatic assays described here should accelerate our mechanistic understanding of quorum-sensing signal synthesis that could pave the way for discovery of potent antivirulence compounds.

Indexed as

2,6-DichloroindophenolAcyl-ButyrolactonesAdenosineChromatography, High Pressure LiquidColorimetryEnzyme AssaysKineticsLigasesReference StandardsSolutionsSpectrophotometry2,6-DichloroindophenolAcyl-ButyrolactonesAdenosineLigasesN-acylhomoserine lactone synthaseSolutions5′-Deoxy-5′-(methylthio)adenosineAcyl carrier proteinAHL synthaseCoenzyme AColorimetric assayHPLC assayMethylthioadenosine nucleosidaseN-acyl-homoserine lactoneQuorum sensingS-adenosyl-L-methionineSpectrophotometric assayXanthine oxidase

Identifiers

PMID29130172

DOI10.1007/978-1-4939-7309-5_13

PMCPMC5766357

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.