ArticleNature communications2017
Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- A pilot study for whole proteome tagging ineLife · 2026Article
- A pilot study for whole proteome tagging inbioRxiv : the preprint server for biology · 2026Article
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Metalens-Based Dual Light-Sheet Fluorescence Microscopy.Small methods · 2025Article
- Fast-adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging.Nature methods · 2025Article
- A Single Sub-Millimetric Metasurface-Based Optical Element for Lattice Bessel Beam Excitation Enabling Brain Activity Recordings In Vivo.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy.Nature communications · 2025Article
- Article
- Live-cell imaging powered by computation.Nature reviews. Molecular cell biology · 2024Review
- Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM).bioRxiv : the preprint server for biology · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
- Targeting N-linked Glycosylation for the Therapy of Aggressive Lymphomas.Cancer discovery · 2023Article
- Practical considerations for quantitative light sheet fluorescence microscopy.Nature methods · 2022Review
- Incorporating the image formation process into deep learning improves network performance.Nature methods · 2022Article
- Multiscale fluorescence imaging of living samples.Histochemistry and cell biology · 2022Review
- Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system.Biomedical optics express · 2022Article
- Multiview confocal super-resolution microscopy.Nature · 2021Article
- Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution.Light, science & applications · 2021Article
- Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms.Scientific reports · 2021Article
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Authors and funding
14 authors.
Funding
Abstract
Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and gentle imaging of live specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolution and collection efficiency of LSFM without modifying the underlying microscope. By imaging samples on reflective coverslips, we enable simultaneous collection of four complementary views in 250 ms, doubling speed and improving information content relative to symmetric dual-view LSFM. We also report a modified deconvolution algorithm that removes associated epifluorescence contamination and fuses all views for resolution recovery. Furthermore, we enhance spatial resolution (to <300 nm in all three dimensions) by applying our method to single-view LSFM, permitting simultaneous acquisition of two high-resolution views otherwise difficult to obtain due to steric constraints at high numerical aperture. We demonstrate the broad applicability of our method in a variety of samples, studying mitochondrial, membrane, Golgi, and microtubule dynamics in cells and calcium activity in nematode embryos.
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