Evidence map›Paper›PMID 29127663›Full record

ArticleInflammation2018

Poly(I:C) Priming Exacerbates Cecal Ligation and Puncture-Induced Polymicrobial Sepsis in Mice.

Deepika Sharma, Ankit Malik, Nandakumar Packiriswamy, Michael D Steury, Narayanan Parameswaran

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Deepika SharmaDepartment of Physiology, Michigan State University, 567 Wilson Road, #2201 Biomedical Physical Sci. Bldg, East Lansing, MI, 48824, USA.
Ankit MalikDepartment of Physiology, Michigan State University, 567 Wilson Road, #2201 Biomedical Physical Sci. Bldg, East Lansing, MI, 48824, USA.
Nandakumar PackiriswamyDepartment of Physiology, Michigan State University, 567 Wilson Road, #2201 Biomedical Physical Sci. Bldg, East Lansing, MI, 48824, USA.
Michael D SteuryDepartment of Physiology, Michigan State University, 567 Wilson Road, #2201 Biomedical Physical Sci. Bldg, East Lansing, MI, 48824, USA.
Narayanan ParameswaranDepartment of Physiology, Michigan State University, 567 Wilson Road, #2201 Biomedical Physical Sci. Bldg, East Lansing, MI, 48824, USA. narap@msu.edu.ORCID http://orcid.org/0000-0002-8894-5564
Michigan State University · US

Funding

Mechanistic basis of probiotic prevention of osteoporosisR01AT007695 · NCCIH · MICHIGAN STATE UNIVERSITY · PI BRITTON, ROBERT A, MCCABE, LAURA R · 2013 to 2017
$2.2M
GPCR Kinase-2 in TNFalpha Signaling in MacrophagesR01HL095637 · NHLBI · MICHIGAN STATE UNIVERSITY · PI PARAMESWARAN, NARAYANAN · 2009 to 2013
$1.8M
Arrestins in TLR4 Signaling in MacrophagesR01AR056680 · NIAMS · MICHIGAN STATE UNIVERSITY · PI PARAMESWARAN, NARAYANAN · 2009 to 2013
$1.4M
The Role of the Gut and Bone Microenvironment in T1D Bone LossR01DK101050 · NIDDK · MICHIGAN STATE UNIVERSITY · PI MCCABE, LAURA R, PARAMESWARAN, NARAYANAN · 2014 to 2016
$1.1M
GPCR Kinase-5 in Inflammatory Bowel DiseaseR21AI099404 · NIAID · MICHIGAN STATE UNIVERSITY · PI PARAMESWARAN, NARAYANAN · 2013 to 2014
$397k
NCCIH NIH HHS R01 AT007695NHLBI NIH HHS R01 HL095637NIAID NIH HHS R21 AI099404NIAMS NIH HHS R01 AR056680NIDDK NIH HHS R01 DK101050NIH HHS HL095637, AI099404, AR056680
6 · The paper itself

Abstract

Sepsis continues to be a major healthcare issue with one of the highest mortality rates in intensive care units. Toll-like receptors are pattern recognition receptors that are intricately involved in the pathogenesis of sepsis. TLR3 is a major receptor for double-stranded RNA and is largely associated with immunity to viral infection. In this study, we examined the role of TLR3 priming in the immunopathology of sepsis using cecal-ligation and puncture (CLP) model of sepsis in mice. Mice injected with vehicle or poly(I:C) were subjected to sham or CLP surgery and various parameters of sepsis, including mortality, inflammation, and bacterial clearance were assessed. Poly(I:C) pre-treatment significantly enhanced mortality in mice subjected to CLP. Consistent with this, inflammatory cytokines including TNFα, IL-12p40, IFNγ, and MCP-1 were enhanced both systemically and locally in the poly(I:C)-treated group compared to the vehicle control. In addition, bacterial load was significantly higher in the poly(I:C)-treated septic mice. These changes were associated with reduced macrophage activation (but not neutrophils) in the peritoneal cavity of poly(I:C) pre-treated mice compared to vehicle pre-treatment. Together our results demonstrate that poly(I:C) priming in sepsis is likely to be detrimental to the host due to effects on systemic inflammatory cytokines and bacterial clearance.

Indexed as

AnimalsBacterial LoadCecumCytokinesDisease Models, AnimalDisease ProgressionInflammation MediatorsLigationMacrophage ActivationMacrophagesMice, Inbred C57BLNeutrophilsPoly I-CPuncturesSepsisTime FactorsCytokinesInflammation MediatorsPoly I-CCecal ligationMortalityPeritoneal infectionPoly(I:C)PolymicrobialPunctureSepsisTLR3 priming

Identifiers

PMID29127663
PMCPMC5764795
OpenAlexW2767413633

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.