Evidence map›Paper›PMID 29127659›Full record

ArticleBotanical studies2017

Characterization of LhSorTGA2, a novel TGA2-like protein that interacts with LhSorNPR1 in oriental hybrid lily Sorbonne.

Le Wang, Zhihong Guo, Yubao Zhang, Yajun Wang, Li Wang, Guo Yang, Wenmei Li, Ruoyu Wang, Zhongkui Xie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Botanical studies, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 20 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Le WangGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Zhihong GuoGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Yubao ZhangGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Yajun WangGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Li WangThe Forest Tree Seedling Station of the Alxa League, Alxa League, 750300, China.
Guo YangGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Wenmei LiGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Ruoyu WangGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Zhongkui XieGaolan Station of the Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China. wxhcas@lzb.ac.cn.
Northwest Institute of Eco-Environment and Resources · CNChinese Academy of Sciences · CNUniversity of Chinese Academy of Sciences · CN

Funding

CAS Key Technology Talent Program 2016-65China Postdoctoral Science Foundation project 2015M580894National Natural Science Foundation of China 31101190National Natural Science Foundation of China 31201651Ningxia Agricultural Comprehensive Development Office NTKJ2015-05-01the Hundred Talents Programs of Chinese Academy of Sciences 27Y127L41002the Hundred Talents Programs of Chinese Academy of Sciences Y629721002
6 · The paper itself

Abstract

backgroundNon-expressor of pathogenesis-related genes 1 (NPR1) regulates expression of pathogenesis-related (PR) genes by interacting with TGA family proteins during systemic acquired resistance (SAR). However, no TGA-like proteins or their interacting partners have been characterized in lily.

resultsIn the present study, LhSorTGA2, a novel TGA-like protein, was identified as an interacting partner of LhSorNPR1 (an NPR-like protein) by bimolecular fluorescence complementation (BIFC) and yeast two-hybrid assay (Y2H). Subcellular localization of GFP-tagged proteins targeted LhSorTGA2 to the nucleus, whereas GFP-labeled LhSorNPR1 was observed both in the nucleus and at the cytomembrane. Sequence alignment revealed that LhSorTGA2 was featured with a basic leucine zipper (bZIP) domain and two glutamine rich acid domains (QI and QII). Further phylogenetic analysis showed that TGA family proteins can be grouped into three subclades, within which LhSorTGA2 was clustered into subclade I, together with AtTGA2/5/6. Expression of LhSorTGA2 was investigated in different tissues by qPCR, and the highest expression level was observed in stem. Besides, when treated with phytohormones (SA, MeJA, ETH and ABA) or fungal pathogen Botrytis elliptica, LhSorTGA2 expression was also induced at different time points post treatments.

conclusionsCollectively, these results suggested that LhSorTGA2 was an interacting partner of LhSorNPR1, which might function in regulating expression of PR genes in lily during SAR.

Indexed as

Gene expressionLilium spp.Non-expressor of pathogenesis-related genes 1Protein interactionSubcellular localizationTGA-like proteins

Identifiers

PMID29127659
PMCPMC5681460
OpenAlexW2767210810

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