ArticleScientific reports2017
Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- That Stinging Sensation: Modularity and the Origin of the Stinging Cell.Integrative and comparative biology · 2025Review
- Lysine myristoylation mediates long-term potentiation via membrane enrichment of synaptic plasticity effectors.The EMBO journal · 2025Article
- Identification of the role of SNARE proteins in rAAV vector production through interaction with the viral MAAP.Molecular therapy. Methods & clinical development · 2025Article
- LRO biogenesis and function: what can we learn from mast cells?Frontiers in cell and developmental biology · 2025Review
- Prediction of Rab5B inhibitors through integrative in silico techniques.Molecular diversity · 2024Article
- SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.Communications biology · 2024Article
- SNAP23 decreases insulin secretion by competitively inhibiting the interaction between SNAP25 and STX1A.Bioscience reports · 2023Article
- Endosomal Rab GTPases regulate secretory granule maturation inCommunicative & integrative biology · 2021Article
- Cytoskeletal Transport, Reorganization, and Fusion Regulation in Mast Cell-Stimulus Secretion Coupling.Frontiers in cell and developmental biology · 2021Review
- Regulatory Mechanism of SNAP23 in Phagosome Formation and Maturation.Yonago acta medica · 2020Review
- MHC class I and II peptide homology regulates the cellular immune response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- An early endosome-derived retrograde trafficking pathway promotes secretory granule maturation.The Journal of cell biology · 2020Article
- Simultaneous Release of Multiple Vesicles from Rods Involves Synaptic Ribbons and Syntaxin 3B.Biophysical journal · 2020Article
- Mammalian diaphanous-related formin 1 (mDia1) coordinates mast cell migration and secretion through its actin-nucleating activity.The Journal of allergy and clinical immunology · 2019Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
Compound exocytosis is considered the most massive mode of exocytosis, during which the membranes of secretory granules (SGs) fuse with each other to form a channel through which the entire contents of their granules is released. The underlying mechanisms of compound exocytosis remain largely unresolved. Here we show that the small GTPase Rab5, a known regulator of endocytosis, is pivotal for compound exocytosis in mast cells. Silencing of Rab5 shifts receptor-triggered secretion from a compound to a full exocytosis mode, in which SGs individually fuse with the plasma membrane. Moreover, we show that Rab5 is essential for FcεRI-triggered association of the SNARE protein SNAP23 with the SGs. Direct evidence is provided for SNAP23 involvement in homotypic SG fusion that occurs in the activated cells. Finally, we show that this fusion event is prevented by inhibition of the IKKβ2 kinase, however, neither a phosphorylation-deficient nor a phosphomimetic mutant of SNAP23 can mediate homotypic SG fusion in triggered cells. Taken together our findings identify Rab5 as a heretofore-unrecognized regulator of compound exocytosis that is essential for SNAP23-mediated granule-granule fusion. Our results also implicate phosphorylation cycles in controlling SNAP23 SNARE function in homotypic SG fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.